2023-2024 / BIOC9243-1

Equilibria in biochemistry and enzime kinetics


20h Th, 40h Pr

Number of credits

 Bachelor in biology4 crédits 


André Matagne

Language(s) of instruction

French language

Organisation and examination

Teaching in the second semester


Schedule online

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

In its first part, this course highlights the importance of equilibria in biochemistry, whereas the second part is an introduction of some fundamental aspects in enzyme kinetics. The first part is organized as follows: 1) Chemical equilibria; 2) Acid-base equilibria; 3) Complexation equilibria. As for the second part: 1. General properties of enzymes; 2. Steady-state kinetics; 3. Enzyme inhibition. More in depth analysis is covered in the Master course entitled "Enzymology".

Learning outcomes of the learning unit

The aim of this course is to familiarize students with equilibria in biochemistry and also with some fundamental aspects of enzymology.

Prerequisite knowledge and skills

Fundamentals of mathematics, chemistry and biochemistry.

Planned learning activities and teaching methods

The first part of the course describes some important aspects relating to equilibria in biochemistry. The second part consists in an introduction to enzyme kinetics. Witten exercises and practical courses are full part of the teaching.

Mode of delivery (face to face, distance learning, hybrid learning)

Face-to-face course

Additional information:

Most lectures are given using the blackboard and white chalk. Problem tutorials are organized in relation with some chapters. Finally, 40 h of practical course are dedicated to protein purification and enzyme catalysis.

Recommended or required readings

Lecture notes are distributed as the course progresses.


Some references:

N.C. Price, R.A. Dwek, R.G. Ratcliffe and M.R. Wormald, Principles and Problems in Physical Chemistry for Biochemists, Third Edition, Oxford University Press, 2001.

K.E. van Holde, W. C. Johnson, P.S. Ho, Principles of Physical Biochemistry, Prentice Hall, 1998.

A. Cornish-Bowden, M. Jamin, V. Saks: Cinétique enzymatique, Grenoble Sciences/EDP Sciences, 2005.

A.R. Fersht, Structure and mechanism in protein science, W.H. Freeman and Co, 1999.


Exam(s) in session

Any session

- In-person

written exam ( open-ended questions )

Written work / report

Additional information:

Written exam + practical courses

Work placement(s)

Non applicable

Organisational remarks and main changes to the course

Lectures will be organized according to the schedule known by the students. The professor will inform the students in advance of any possible modification to this


André Matagne, PhD, Professor, Enzymology and Protein Folding, Centre for Protein Engineering, InBioS RU, Life Science Department, Institut de Chimie B6c (room 3/61), Allée de la Chimie, 3, University of Liège, B4000 Liège (Sart-Tilman), Tel.: +32 (0)4 3663419, Email: amatagne@ulg.ac.be

Association of one or more MOOCs