2019-2020 / CHIM0717-1

Microbial processes and interactions

Duration

38h Th, 28h Pr

Number of credits

 Master in bioengineering : chemistry and bio-industries (120 ECTS)7 crédits 

Lecturer

Frank Delvigne, Patrick Fickers, Philippe Jacques, Marc Ongena, Jean-Claude Twizere

Coordinator

Patrick Fickers

Language(s) of instruction

English language

Organisation and examination

Teaching in the second semester

Schedule

Schedule online

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

This course focus on theoretical and practical aspects on technologies related to the production of compounds of biotechnological interest (biomass, proteins, metabolites) for agro-food, chemical and biopharmaceutical industries. The course details the different technologies of bioreactor culture, molecular techniques leading to the development of "cell factory" as well as cell-environment interactions.

Learning outcomes of the learning unit

At the end of this multidisciplinary module, students will be able to integrate the different aspects of the development, implementation, optimization and control of a process of production of a compound of interest in bioreactor. The course also includes dimensioning part (calculation of oxygen transfer capacity, dynamics of microbial growth and production of metabolites, ...) and on the construction of dynamic models of bioprocesses (resolution of first-order differential equations under MatLab).

Prerequisite knowledge and skills

BIOL2010-1 : Biologie cellulaire
BIOL2013-2 : Microbiologie générale - Patrim 1
BIOL2013-3 : Microbiologie générale - Partim 2
BIOL2015-3 : Biologie moléculaire
BIAA0003-1 : Génie industriel alimentaire et ingénierie agro-alimentaire - 1ère partie
CHIM9241-1 : techniques de séparation et de concentration
MATH2016-1 : mathématique générale et modélisation des systèmes dynamiques

Planned learning activities and teaching methods

- Bioreactor engineering (F. Delvigne, 12 h Th)
- Metabolic engineering (P. Fickers, P. Jacques, 12 h Th)
- Microbial interaction (M. Ongena, 12 h Th)
- Animal cell culture (JC Twizere , 12 h Th)
- Exercices (F. Delvigne, P. Fickers, P. Jacques, 12 h, )
- Lab (P. Fickers, P. Jacques, M. Ongena, F. Delvigne, 12 h)
- Numerical simulation (F. Delvigne, 12 h, exercices)
Beside ex-cathedra courses, this module includes practical lab work, exercises (simulation on MatLab), seminars (industrials, academics or research centers) and company visits Active. In this course, a pedagogical approach "learning by doing" will also be favored..

Mode of delivery (face-to-face ; distance-learning)

Lectures
Lab training 
Exercices (numerical simulation using MatLab), 
Seminars 
Company visit
In this course, a pedagogical approach "learning by doing" will also be favored.

Recommended or required readings

Assessment methods and criteria

Report and oral presentation
Oral and writen exam

Work placement(s)

Organizational remarks

Contacts

Prof. Patrick Fickers
Microbial Processes and Interactions (MiPI)
Ph: 081 622 311 (direct), 081 622 305 (secretariat)
pfickers@ulg.ac.be

Adaptation of teaching commitments following the COVID-19 pandemic for the May-June 2020 session

Teaching methods implemented : distance-learning

The lessons and exercise sessions are given remotely via LifeSize using the course notes available on eCampus or sent directly to students (power point files) and according to the schedule included in CelCAT. Some sessions are recorded and the video files are also deposited on eCampus. Exercise resolutions related to modeling are available on a GitLab directory at the following address: https://gitlab.uliege.be/F.Delvigne/bioreactor-modelling-and-systems-biology.

Assessment subjects

The matter for the oral evaluation corresponds to the following parts:

  • From the screening to the formulation of microbial products, the example of lipopeptides (Pr. Philippe Jacques)
  • Bioreactor modeling and system biology (Pr. Frank Delvigne) + Conference "Mass spectrometry with single microbial cell" (Dr Christian Dusny)
  • Microbial Interactions (Pr. Marc Ongena)
The material assessed through written reports corresponds to the following parts:
  • Bioninformatics exercises (Pr. Philippe Jacques)
  • Patent exercises (Pr. Philippe Jacques and F. Piron)
  • Cell cultures exercises (Pr. Jean-Claude Twizere)
The Metabolic engineering of yeast (Pr. Patrick Fickers) part is not part of the subjects of the evaluation.

Assessment methods

Oral exam via LifeSize according to the schedule included in CelCAT. An order of passage and the precise modalities of the exam will be communicated in due time by e-mail via eCampus.
Three written works: bioinformatics work (P. Jacques) submitted for March 29, intellectual property work (P. Jacques) to be submitted by April 30 and article review to be sent by email before June 10, 2020 (JC Twizere).

Contacts

Marc Ongena (marc.ongena@uliege.be) , Frank Delvigne (f.delvigne@uliege.be), Jean-Claude Twizere (jean-claude.twizere@uliege.be), Philippe Jacques (Philippe.jacques@uliege.be).

Adaptation of teaching commitments following the COVID-19 pandemic for the Aug-Sept 2020 session

Assessment subjects

Assessment methods

Contacts

Items online

Interactome networks to understand genotype-phenotype relationships
The slides contain key slides to explain genotype - phenotype relationships, based on the understanding genotype-phenotype relationships. We will focus on protein-protein interactions technologies and give pratical examples.