Application of molecular biology and genetics

Duration

24h Pr

Number of credits

Lecturer

Nicolas Gengler, Ludivine Lassois, Hervé Vanderschuren, Luc Willems

Substitute(s)

Martine Schroyen

Coordinator

Nicolas Gengler

Language(s) of instruction

French language

Organisation and examination

Teaching in the second semester

Schedule

Schedule online

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

The course "Application of molecular biology and genetics" will be devoted to the integrated practical application of the concepts seen in the General genetics and Molecular biology classes. This exercise will include the collection of biological material, the molecular analyze in the laboratory and the interpretation of results.

Learning outcomes of the learning unit

At the end of this class, the student must have assimilated the theoretical bases of the most common techniques of molecular biology and genetics. He should also be able to understand and perform a series of techniques in the laboratory. He should be able to interpret the experimental results obtained during the practical course in the context of genetics.

Prerequisite knowledge and skills

BOTA0414-1 - Botany systematics 
ZOOL2020-1 - Zoology : Evolution
STAT2002-1 - Basic statistics , part 1
BIOL2010-1 - Cell Biology
STAT2003-1 - Basic statistics , part 2
BIOL2015-3 - Molecular Biology

Planned learning activities and teaching methods

The collection of biological material will be done as part of a visit in the field. Molecular analyzes will include a theoretical part describing the main techniques which will then be applied in a practical session. The aim will be to extract and analyze nucleic acids. A last part will include analysis and interpretation of data.

Mode of delivery (face-to-face ; distance-learning)

Face-to-face

Recommended or required readings

- Molecular Biology of the Gene 7th ed. (Watson, Baker, Bell, Gann, Levine, Losik), Pearson International, 2014
- Molecular Biology 2th ed. (Clark and Pazdernik), APCell press, 2013

Assessment methods and criteria

This teaching unit is composed of four parts which are:

• molecular and laboratory part, below entitled "practical part": responsible Prof. Luc Willems,
• genetic and preservation of ligneous biodiversity part: responsible Prof. Ludivine Lassois
• applied crop genetics part: responsible Prof. Hervé Vanderschuren
• populations and quantitative genetics part: responsible Prof. Nicolas Gengler

• equal to the score of the practical part if this mark is lower than the final mark calculated by average
• equal to the final mark calculated by average if it is lower than the mark of the practical part.

Work placement(s)

Organizational remarks

Contacts

Luc Willems  Research Director of the FNRS Professor at ULg Molecular Biology Tel: +32 81 62 21 57 Luc.willems@ulg.ac.be
Hervé Vanderschuren Professor Plant Genetics Gembloux Agro-Bio Tech University of Liège Tel: +32 81 62 25 71 herve.vanderschuren@ulg.ac.be
Ludivine Lassois Forestry genetics University of Liège Gembloux Agro-Bio Tech BIOSE Departement  Passage des Déportés, 2 B-5030 Gembloux Belgium Tel: + 32 81 62 23 21 ludivine.lassois@ulg.ac.be
Nicolas Gengler Professor Numerical genetics, genomics and modeling Gembloux Agro-Bio Tech Université de Liège Tel: +32 81 62 22 06 nicolas.gengler@ulg.ac.be

Adaptation of teaching commitments following the COVID-19 pandemic for the May-June 2020 session

Teaching methods implemented : distance-learning

The practical course scheduled on April 21 is replaced by a DNA extraction to be performed home. A video presenting the laboratory of molecular biology and the DNA extration techniques are uploaded on eCampus.
The sessions of April 24 and 28, and May 6 are maintained. Using tools available on eCampus, computation sessions will be organized remotely. Commented powerpoints, interactive exercises and their corrections, and required teaching materials will be available on e-campus.
All other scheduled sessions will be cancelled to lighten the students' workload.

Assessment subjects

The practical course scheduled on April 21 will be evaluated on the basis of a written report to be submitted on line (eCampus). The work includes a senario and a DNA extraction to be performed home. Details are provided on eCampus.
The ability to do and understand the exercises performed during the computing sessions organized in distance learning and other elements acquired during the virtual excursion and through other interactions with the teachers will be evaluated.

Assessment methods

A report of the DNA extraction and its scenario to be submitted on line on eCampus.
The evaluation of the other parts will be done through a written evaluation using the eCampus tools. The details of the evaluation process will be co-constructed with the students before the evaluation.
The final grade will be established by consultation between the teachers considering the circonstances. No partial waivers will be granted.

Contacts

Adaptation of teaching commitments following the COVID-19 pandemic for the Aug-Sept 2020 session

Assessment subjects

For the student who did not succeed the practicals, a written report has to be submitted on line (eCampus). The work includes a scenario and a DNA extraction to be performed home. Details are provided on eCampus.
The ability to do and understand the exercises performed during the computing sessions organized in distance learning and other elements acquired during the virtual excursion and through other interactions with the teachers will be evaluated.

Assessment methods

A report of the DNA extraction and its scenario to be submitted on line on eCampus.
The evaluation of the other parts will be done through a written evaluation using the eCampus tools. 
The final grade will be established as the average between the two parts. Partial waivers based on the first session will be granted.

Contacts