Techniques for cells and tissue cultures

Duration

30h Th

Number of credits

Lecturer

Julie Lecomte, Erik Maquoi

Coordinator

N...

Language(s) of instruction

French language

Organisation and examination

Teaching in the first semester, review in January

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

The course is subdivided in the following way: - cellular lines - techniques of aseptie - environment of the culture - primary cultures - techniques of observation in optical  - cloning, and selection of the cellular types - characterization - the transformed phenotype - contaminations - instability, variation and conservation - quantification and experimental projects - measurement of viability and cytotoxicity - three-dimensional culture - biocompatibility and toxicity

Learning outcomes of the learning unit

The philosophy of the course of culture of cells is to communicate to the students the basic techniques used for the culture of animal or human cells in vitro

The objective continued are:
- maintenance of the viable isolated cells or the functional units apart from the organism;
- application of good laboratory practices;
- handling, which constitutes the best training for the student.

Prerequisite knowledge and skills

Good knowledge in cellular and tissue biology

Planned learning activities and teaching methods

- Preparation of sterilized material and media - Starting cells culture - Congelation -decongelation - Cells count - Passaging - Viability tests - Observations (phase contrast and fluorescence microscopies) - Mycoplasma detection
Local: 3/62 - Institute of chemistry (B6c)

Mode of delivery (face-to-face ; distance-learning)

First semester Course: face-to-face TP: in L2 Laboratory

Recommended or required readings

Syllabus: Culture de cellules - Presses universitaires de Liège Support DVD for workshop: La culture cellulaire Notes de travaux pratiques DIAs ( power point presentation) Presses universitaires  de Liège
Cours and notes also on line

Assessment methods and criteria

Practical : - Laboratory book (/20) - 15 december - Workshop (/20) - december
Theory: written examination in january (/60) To be modified in function of the teaching duties

Work placement(s)

Organizational remarks

Blouse of laboratory obligatory
Laboratory book

Contacts

Dr. Erik Maquoi & Dr. Julie Lecomte
Laboratoire de Biologie des Tumeurs et du Développement
Tour de Pathologie, B23 (+4), route 688
Avenue Hippocrate, 13 Sart - Tilman B 4000 Liège Tél: 04/366.25.31 E-mail: Erik.Maquoi@uliege.be / jlecomte@uliege.be

Items online