Duration
15h Th
Number of credits
| Master in chemistry (120 ECTS) | 2 crédits |
Lecturer
Language(s) of instruction
French language
Organisation and examination
Teaching in the first semester, review in January
Units courses prerequisite and corequisite
Prerequisite or corequisite units are presented within each program
Learning unit contents
The course is a practical application of NMR techniques studied in the course on Structural Analysis (CHIMI0650).
After a lesson summarizing the theoretical principles and practicalities of different NMR experiments, students will be asked to record themselves their own experiences protein in solution interacting with ligands.
The proposed experiments are the following 1D 1H with water suppression, 2D HSQC (HN).
Learning outcomes of the learning unit
Objective 1: Acquiring the ability to record adequately all NMR experiments required for the analysis of biological macromolecule in water Objective 2: To understand the spectral information obtained by applying a sequence of pulses. Objective 3: to choose experiences tailored to their research.
Prerequisite knowledge and skills
Course on structural analysis: CHIM-0213 Course on Structural biological Chemistry CHIM-0650
Planned learning activities and teaching methods
Practical work on the NMR spectrometer. Preparation of the biological samples. Analysis of the spectra with appropriate computer programs : TOPSPIN
Mode of delivery (face-to-face ; distance-learning)
The course schedule will be determined with the students according to the availabilities of each and the availability of the spectrometer.
Recommended or required readings
course CHIM-0213 (structural analysis) cours CHIM-0650 (structural biological chemistry )
Assessment methods and criteria
Written report on the analysis performed by the students.
Work placement(s)
Organizational remarks
Contacts
C. Damblon (C.Damblon@ulg.ac.be)