Duration
20h Th
Number of credits
| Master in bio-informatics and modelling (120 ECTS) | 3 crédits | |||
| Master in biochemistry and molecular and cell biology (120 ECTS) | 3 crédits |
Lecturer
Frédéric Bouché, Alain Brans, Franck Dequiedt, Mireille Dumoulin, Yvette Habraken, Sylvie Legrand, Isabelle Manfroid, N..., H. Pendeville-Samain, Mohammed Terrak, Marianne Voz
Coordinator
Language(s) of instruction
French language
Organisation and examination
Teaching in the first semester, review in January
Schedule
Units courses prerequisite and corequisite
Prerequisite or corequisite units are presented within each program
Learning unit contents
1. The gene - Enzymatic manipulation of DNA - Cloning - Vectors used in prokaryotes - Bacterial hosts
2. Heterologous expression in Gram-negative bacteria - Production of recombinant proteins and gene fusions
3. Heterologous expression in Gram-positive bacteria - Molecular biology and genetic manipulation of yeasts.
4. Cosmids, phagemids and phage display, methodologies and applications.
5. Mutagenesis techniques and their applications - Site-targeted mutagenesis - Saturation mutagenesis - Directed evolution.
6. Plant genetic engineering: transformation methods - Agrobacterium tumefaciens - Stable transgenesis & agroinfiltration - Application examples.
7. Expression of proteins in mammalian cells - Transfection techniques - Vector viruses - siRNA / shRNA.
8. Detection of protein-protein interactions (Two-hybrid system).
9. Genome editing / Transgenesis.
10. Genome editing / Transgenesis (applications).
Learning outcomes of the learning unit
· To get acquainted with the main methods used in genetic engineering
· To get informed of the potential of genetic engineering in the study of living organisms, as well as in the improvement of species for human use and for the production of recombinant proteins.
Prerequisite knowledge and skills
Molecular biology and genetics (courses of the bachelier in Biology).
Planned learning activities and teaching methods
Mode of delivery (face to face, distance learning, hybrid learning)
Face-to-face course
Additional information:
This course concerns only students who attended it but did not pass the exam during the academic year 2022-2023. From 2023-2024, there will be no face-to-face lectures. Questions/answers sessions will be organized on request.
Recommended or required readings
Powerpoint presentations and lecture texts available through the eCampus system.
Exam(s) in session
Any session
- In-person
written exam
Additional information:
Written exam (first exam session, as well as second one).
Work placement(s)
Organisational remarks and main changes to the course
Contacts
- Sylvie Legrand (S.Legrand@uliege.be)
- Mohammed Terrak (mterrak@uliege.be)