2017-2018 / CHIM0627-1

Structure of biological macromolecules (experimental aspects) : part b1 (RX, NMR)

Duration

15h Th, 10h Pr

Number of credits

 Master in bio-informatics and modelling (120 ECTS)3 crédits 

Lecturer

Paulette Charlier, Christian Damblon

Coordinator

Paulette Charlier

Language(s) of instruction

French language

Organisation and examination

Teaching in the first semester, review in January

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

The course goes into deeper description of of the techniques used to investigate the structure of proteins: X-ray Crystallography and Nuclear Magnetic Resonance.

I- X-ray diffraction (P. Charlier) Theoretical part: 1- High resolution structure, 2- Neutron diffraction, 3- Laue diffraction Practical part: Solving of a protein structure in silico on the basis of X-ray diffraction data.
II- Nuclear Magnetic Resonance (C. Damblon) Theoretical part: 1- Protein-ligand interactions Practical part: Data collection with a 500MHz spectrometer.

Learning outcomes of the learning unit

This course gives to the future bioinformaticians the bases necessary to understand the principles of determination of 3D protein structure by X-ray crystallography and nuclear magnetic resonance in order to enable them to make an objective use of the models. It gives them an outline of the capacities and limits of various techniques.

Prerequisite knowledge and skills

Course of physics of 1st cycle. Basic knowledge in X-ray crystallography. Good knowledge of the basic principles of the structures of proteins. Biophysical course.

Planned learning activities and teaching methods

Tutorials dedicated to the Pymol software use: obseravtion and analysis of macromolecules structures. Use of PDB database. Solving of a protein structure in silico on the basis of X-ray diffraction data.

Mode of delivery (face-to-face ; distance-learning)

To be decided in concertation with the different lecturers.

Recommended or required readings

The PowerPoint presentations of the theoretical course will be available on CD as well as the notes of practical works. Books recommended: - "Crystallography made hook clear: With guide for users for macromolecular models." Scale Rhodos, Academic Press 2000 - "Introduction to Protein Structure" Carl Branden and John Tooze, Garland Publishing 1999 CD roms: - PS ² and Kinemage Supplement to "Introduction to Protein Structure" Carl Branden and John Tooze, Garland Publishing 1999

Assessment methods and criteria

To be decided in concertation with the different lecturers. Most probably on the basis of a research article.

Work placement(s)

Organizational remarks

Contacts

Paulette Charlier, PhD, Chargé de CoursCristallographie des macromolécules biologiques, Centre d'Ingénierie des Protéines, Département des Sciences de la Vie, Université de Liège, Institut de Physique, B5a, B-4000 Liège, Belgium Tel: +32 (0)4 366 36 19 Fax: +32 (0)4 366 49 54 E-mail : Paulette.Charlier@ulg.ac.be http://www.cip.ulg.ac.be/
DAMBLON Christian Département de chimie (sciences) / Chimie biologique structurale Bât. B6 Chimie biologique structurale allée du 6 Août 3 4000 Liège 1 Belgique Tél. ULg +32 4 3663788 Fax ULg +32 4 3664577 c.damblon@ulg.ac.be