Duration
20h Th, 40h Pr
Number of credits
| Bachelor in biology | 4 crédits | |||
| Master of education, Section 4: Biology | 4 crédits |
Lecturer
Language(s) of instruction
French language
Organisation and examination
Teaching in the second semester
Schedule
Units courses prerequisite and corequisite
Prerequisite or corequisite units are presented within each program
Learning unit contents
The first part of this course highlights the importance of equilibria in biochemistry and covers the following topics: (1) chemical equilibria; (2) acid-base equilibria; (3) complexation equilibria. The second part introduces fundamental concepts in enzymology and enzyme kinetics: (1) general properties of enzymes; (2) steady-state enzyme kinetics; (3) enzyme inhibition. The topics are explored in greater depth in the master's-lecel course entitled "Biotechnologies".
Learning outcomes of the learning unit
By the end of this course, students will have acquired the fundamental concepts required to study equilibria in biochemistry and enzyme kinetics. They will understand the essential principles of enzyme function and be able to use the main tools employed for their kinetic characterization.
Prerequisite knowledge and skills
Basic knowledge of mathematics, chemistry and biochemistry.
Planned learning activities and teaching methods
The first part of the course, delivered through lectures, focuses on the main factors governing equilibria in biochemistry. The second part provides an introduction to enzyme kinetics. Tutorial sessions and practical laboratory work allow students to apply the concepts acquired during the course to concrete situations.
Attendance at the preparatory session and all practical laboratory sessions is compulsory.
Mode of delivery (face to face, distance learning, hybrid learning)
Face-to-face course
Further information:
Teaching is conducted entirely in person. Most lectures are delivered using the blackboard and white chalk. Tutorial sessions are also organized to allow students to solve practical problems related to selected theoretical topics. Finally, 40 hours of laboratory work are devoted to enzyme kinetics.
Attendance at the introductory laboratory session is mandatory and is a prerequisite for participation in the subsequent practical sessions.
Course materials and recommended or required readings
Platform(s) used for course materials:
- MyULiège
Further information:
Lecture notes are provided progressively as the course advances. They are intended to facilitate the study of the course material, but their use is not compulsory to acquire the learning outcomes of this course.
Recommended reference books:
N.C. Price, R.A. Dwek, R.G. Ratcliffe and M.R. Wormald, Principles and Problems in Physical Chemistry for Biochemists, Third Edition, Oxford University Press, 2001.
K.E. van Holde, W. C. Johnson, P.S. Ho, Principles of Physical Biochemistry, Prentice Hall, 1998.
A. Cornish-Bowden, M. Jamin, V. Saks: Cinétique enzymatique, Grenoble Sciences/EDP Sciences, 2005.
A.R. Fersht, Structure and mechanism in protein science, W.H. Freeman and Co, 1999.
Exam(s) in session
Any session
- In-person
written exam ( open-ended questions )
Written work / report
Further information:
Assessment is based on a written examination, which accounts for 70% of the final grade, and on the work carried out during the practical sessions and the activity report, which together account for 30% of the final grade.
Active, in-person participation in all practical sessions is compulsory. In accordance with Article 61 of the General Study and Examination Regulations, students who fail to meet this requirement will not be allowed to take the assessments organized for this course unit or to obtain the corresponding credits.
Work placement(s)
Non applicable
Organisational remarks and main changes to the course
Classes are held according to the schedule communicated to the students, unless otherwise indicated. Students will be informed in advance of any change to the schedule, including the cancellation of a session, either during class or by email, where appropriate through the class representative.
Contacts
André Matagne, PhD, Full Professor, Enzymology and Protein Folding, Centre for Protein Engineering, InBioS RU, Life Science Department, Institut de Chimie B6c (room 3/1), Allée de la Chimie, 3, University of Liège, B4000 Liège (Sart-Tilman), Tel.: +32 (0)4 3663419, Email: amatagne@uliege.be (to be preferred)