2023-2024 / BIOC0721-1

Optical spectroscopy for biochemistry


15h Th

Number of credits

 Master in bio-informatics and modelling (120 ECTS)2 crédits 
 Master in biochemistry and molecular and cell biology (120 ECTS)2 crédits 


Christian Damblon, André Matagne


André Matagne

Language(s) of instruction

French language

Organisation and examination

Teaching in the first semester, review in January


Schedule online

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

The course presents various optical methods used in biochemistry.
Spectroscopic methods taught in the course are : UV-Visible absorption, linear and circular dichroisms, fluorescence, vibrational spectroscopy (i.e. infrared and raman), surface plasmon resonance and biolayer interfrometry.
For each method, principles, technical aspects and applications focused on biological macromolecules will be developed.
The course will concludes with a review of the optical properties of proteins.

Learning outcomes of the learning unit

Increase the student skills regarding the available spectroscopic methods for the study of the conformational dynamics of biological macromolecules.

Prerequisite knowledge and skills

Basic chemistry and physics

Planned learning activities and teaching methods

Lectures are given with the help of powerpoint presentations, blackboard and chalk

Mode of delivery (face to face, distance learning, hybrid learning)

Face-to-face course

Additional information:

Due to the 2023-2024 reform of the BBMC Master, this course is no longer organised and is only accessible to students who did not pass the exam before the reform started.

Recommended or required readings

Powerpoint presentations or lecture notes will be available following the lectures Reference books: - Biophysical Chemistry, Cantor and Schimmel - Principles of Physical Chemistry, Van Holde et al. - Biological Spectroscopy, Campbell and Dwek - Physical Biochemistry, Sheehan

Exam(s) in session

Any session

- In-person

written exam

Additional information:

Student evaluation will be based on a written or oral exam. Students will be informed in due time.


Work placement(s)

Non applicable

Organisational remarks and main changes to the course

Lectures will be organized following the schedule known by the students. The professor will inform the students in advance of any possible modification to this


Christian Damblon, Professor, Department of Chemistry, Institut de Chimie B6c, room 3/71, Allée de la Chimie, 3, University of Liège, B4000 Liège (Sart-Tilman), Tel.: +32 (0)4 3663788, Fax: +32 (0)4 3663413, E-mail: c.damblon@ulg.ac.be

André Matagne, PhD, Full Professor, Enzymology and Protein Folding, Centre for Protein Engineering, Life Science Department, Institut de Chimie B6c, room 3/61, Quartier Agora, Allée du 6 Août, 13, University of Liège, B4000 Liège (Sart-Tilman), Tel.: +32 (0)4 3663419, Email: amatagne@ulg.ac.be

Association of one or more MOOCs