2023-2024 / BIOC0210-5



20h Th, 10h Mon. WS

Number of credits

 Master in bio-informatics and modelling (120 ECTS)3 crédits 
 Master in biochemistry and molecular and cell biology (120 ECTS)3 crédits 


André Matagne

Language(s) of instruction

French language

Organisation and examination

Teaching in the first semester, review in January


Schedule online

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

This course is organized as follows: Chapter 1: General properties of enzymes. Chapter 2: Steady-state kinetics. Chapter 3: Enzyme inhibition. Chapter 4: Effect of pH, temperature, viscosity and other experimental factors. Chapter 5: Reactions of two substrates. Chapter 6: Transient-state kinetics. Chapter 7: Cooperativity and allosteric interactions. Chapter 8: Enzyme inactivators. Chapter 9: Enzyme cofactors. Chapter 10: Mechanism of chymotrypsin. Chapter 11: Basic catalytic principles. Chapter 12: Regulation of catalytic activity.

In addition, a few sessions will be dedicated to specific topics (e.g. nanobodies as enzyme inhibitors, enzymes of the peptidoglycan synthesis pathway, the formation of disulfide bridges in proteins), taught by specialists. These sessions aim to illustrate the great diversity of biolochemical reactions catalyzed by enzymes.

Learning outcomes of the learning unit

The aim of the course is to make students familiar with enzyme mechanisms and fundamentals of enzyme kinetics.

Prerequisite knowledge and skills

Course on "Equilibriums in biochemistry and enzyme kinetics" (BAC Biology), which includes an introduction to enzyme catalysis (chapters 1-3; note that both theoretical and practical aspects are covered).

Planned learning activities and teaching methods

Most lectures are given using the blackboard and white chalk. Problems tutorials are organized in relation with some chapters.

Mode of delivery (face to face, distance learning, hybrid learning)

Face-to-face course

Additional information:

Due to the 2023-2024 reform of the BBMC Master, this course is no longer organised and is only accessible to students who did not pass the exam before the reform started.

Recommended or required readings

Lecture notes will be available as the course progresses.
Reference book:
A. Cornish-Bowden, Fundamentals of enzyme kinetics, fourth edition, Wiley-Blackwell, 2012.
A.R. Fersht: Structure and mechanism in protein science, W.H. Freeman and Co, 1999.

Exam(s) in session

Any session

- In-person

written exam ( open-ended questions )

Additional information:

Written exam

Work placement(s)

Non applicable

Organisational remarks and main changes to the course

Lectures will be organized according to the schedule known by the students. The professor will inform the students in advance of any possible modification to this


André Matagne, PhD, Full Professor, Enzymology and Protein Folding, Centre for Protein Engineering, Life Science Department, Institut de Chimie B6c (room 3/61), Quartier Agora, Allée du 6 Août, 13, University of Liège, B4000 Liège (Sart-Tilman), Tel.: +32 (0)4 3663419, Email: amatagne@ulg.ac.be

Association of one or more MOOCs