Duration
20h Th, 40h Pr
Number of credits
| Bachelor in biology | 5 crédits |
Lecturer
Language(s) of instruction
French language
Organisation and examination
Teaching in the first semester, review in January
Schedule
Units courses prerequisite and corequisite
Prerequisite or corequisite units are presented within each program
Learning unit contents
First part: The concepts of analytical chemistry and the importance of analytical chemistry in biology and life science: 1. Introduction 2. Measurements in analytical chemistry 3. Experimental errors Second part : The reactions and chemical equilibrium, their role in biochemistry and their role to develop analytical methods 4. Chemical equilibria 5. Titrations 6. Acid-Base equilibria 7. Complexation equilibria Third part: introduction to separation methods. We discuss the basic principles of chromatographic separation mechanism. We will detail the different parameters that characterize a chromatographic separation. We discuss the main types of chromatography: Gas chromatography Liquid chromatography Ionic chromatography Size exclusion chromatography
Learning outcomes of the learning unit
To understand, interpret and apply the various reaction steps of an analysis protocol in order to obtain the best performances of accuracy and precision are the top priority of the course. This implies a perfect knowledge of the chemical reactions which it involves.The understanding of coupled chemical equilibria is another learning outcome indispensable for future biochemistry courses. In the third part, understanding the basics in chromatography imply a perfect knowledge of separation mechanisms involved. The students will be able to select the adequate chromatographic technique including the selection of the stationary and mobile phases to separate biomolecules. They will also be able to understand the role that experimental parameters play on the efficiency of a chromatographic separation.
Prerequisite knowledge and skills
The general chemistry course of the first year
Planned learning activities and teaching methods
See below
Mode of delivery (face to face, distance learning, hybrid learning)
Theoretical course: The lessons (2h) begin the first Tuesday of the first quadrimester from 10h30 to 12h30 (see Celcat). The course uses powerpoint presentations and exercices on the blackboard. An interruption of 5 to 10 minutes is provided at about the middle of each lesson. Due to Covid crisis, the courses are given alternatively for the students in face to face and visoconferences according to the recommendations given.
Directed works:
Six sessions are planned for solving numerical problems in the course material. These sessions are very important because the final written exam is predominantly based on numerical exercises.
Laboratory works: they begin first Wednesday of the first quadrimester of the year (rooms R4 and R10, Chemistry building B6d -TP). They start at 10h until 18h (with an interruption for lunch between 13h and 14h) . The purpose is primarily to initiate the students with the specific requirements of the chemical analysis: do not lose anything, do not contaminate, great care, cleanliness and method, strict respect of the experimental protocols... The reliability, precision, trueness and accuracy of an analysis result depend, of course, on the choice of the method but also on the dexterity and competence of the experimentalist.
Organisational adjustments related to the current health context
Recommended or required readings
The notes as well as the handbook of laboratory work are available at the presses universitaires. The notes alqo include a list of numerical exercises, some solved. No particular material (except the apron) is necessary for the laboratories. The use of a handheld scientific calculator (programmable) is allowed, even advised for the solution of the exercises. As supplementary information, the consultation of the following work is highly recommended: - D.C. Harris, Quantitative Chemical Analysis, 6th or 7th edition, WH Freeman, New York, 2003 for the 6th edition.
Assessment methods and criteria
Below you will find information on the evaluation methods planned for in-person and remote exams as well as those planned for hybrid sessions. Depending on how the health crisis evolves, the chosen method will be communicated to you no later than one month before the start of the exam session.
Practical laboratory work: the evaluation is made on the exactitude of the returned analysis results as well as on the theoretical understanding of the proposed protocols. The final practical works score is calculated on the basis of the experimental reports, the quizzes, and a general written test. They count for 30% of the final grade. From one year to the next, students who graded more than 10/20 do not have to repeat the practical work.
En présentiel
Matière de l'évaluation
L'évaluation portera sur l'ensemble de la matière vue au cours théorique (powerpoints + notes de cours), sur les concepts vus en séances de travaux pratiques ainsi que sur les exercices résolus en séances d'exercices (répétitions), au cours théorique et sur les exercices supplémentaires du fascicule de répétitions.
Méthodes d'évaluation
L'examen écrit sera composé d'une série de questions de théorie, de QCM et de résolutions d'exercices. Sa durée, encore à définir, n'excédera pas 4h00. L'examen représentera 70% de la cote finale, la cote des travaux pratiques en représentera 30%.
En distanciel
Matière de l'évaluation
L'évaluation portera sur l'ensemble de la matière vue au cours théorique (powerpoints + notes de cours), sur les concepts vus en séances de travaux pratiques ainsi que sur les exercices résolus en séances d'exercices (répétitions), au cours théorique et sur les exercices supplémentaires du fascicule de répétitions.
Méthodes d'évaluation
L'examen écrit aura lieu à distance via une plateforme qui sera précisée ultérieurement. Il sera composé d'une série de questions de théorie, de QCM et de résolutions d'exercices. Sa durée, encore à définir, n'excédera pas 4h00. L'examen représentera 70% de la cote finale, la cote des travaux pratiques en représentera 30%.
Certains exercices pourront nécessiter un envoi, par voie électronique, de la résolution manuscrite de l'étudiant, scannée ou photographiée avec une qualité suffisante, avant l'heure de clôture de l'examen. Les étudiants ne disposant pas d'un ordinateur et/ou d'une connexion internet stable sont priés de rapidement prendre contact avec Wendy Müller (wmuller@uliege.be)
Des informations supplémentaires seront prochainement communiquées aux étudiants.
Work placement(s)
Two types of trainings are scheduled:
1- Traditional laboratory sessions are organized during 8 weeks, Wednesdays from 10am to 18pm. Three experiments will be performed (quantitation by gravimetry, colorimetry, and volumetric titration). The students submit a report on their analysis. Quizzes can be organized during the labs to test knowledge and understanding of the present experiments.
2- An observatory training in a research laboratory will be organized to demonstrate the use of a contemporary method or a specific instrument in a biological analytical chemistry laboratory.(CANCELLED THIS YEAR FOR COVID REASON)
These two activities are parts of the teaching module and are mandatory. The student who will not participate at both entire activities and/or provided their reports outside the deadlines fixed will not be admitted at the exam.
Organizational remarks
See here below
Contacts
Pr. Gauthier Eppe
Institut de Chimie, Bat B6c, Local 1/9A
Tel. +32-4-366.3422
e-mail. g.eppe@uliege.be
Exercises sessions: Wendy Müller: wmuller@uliege.be
Laboratories : Damien Coibion: Damien.coibion@uliege.be
Laboratory assistant: : Stéphane Luts