2020-2021 / BIOL0860-1

Soil ecotoxicology and bioremediation

Duration

24h Th, 12h Pr

Number of credits

 Master in biology of organisms and ecology (120 ECTS)3 crédits 

Lecturer

Monique Carnol, Marc Hanikenne, Célia Joaquim-Justo

Language(s) of instruction

French language

Organisation and examination

Teaching in the first semester, review in January

Schedule

Schedule online

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

Course description
Ecotoxicology studies the effects of chemicals on organisms in the environment, with the final aim to protect the structure and functioning of ecosystems. We will address specific issues related to soil ecotoxicology, bioremediation and phytoremediation.
Microorganisms are responsible for the transformation of numerous substances and their activity can be exploited for applied issues, such as bioremediation. In this course, students will learn about interactions between microorganisms, biofilm formation, and microbial processes in the degradation of pollutants (bioremediation). An overview of procedures used for soil depollution will be provided and some case studies will be analysed.
Plants colonize very diverse environments and their root system explores and interacts with soils. Plants are therefore exploited for environmental clean up (phytoremediation). In this course, we will focus on metallic pollution. The metal homeostasis mechanisms present in plants will be discussed and the biology of peculiar plant species adapted to metal pollution will be examined. Different strategies, exploiting biotechnology or plant natural diversity, for phytoremediation of polluted soils and their applications in ongoing field assays will be presented.
The assessment of risks related to the use of toxic pollutants for terrestrial ecosystems is mainly based on the evaluation of their impact on soil organisms and seedlings. The ecological reasons for this strategy used at the EU level together with the common tests used will be reviewed and explained to the students.

Practicals will focus on techniques useful for soil ecotoxicological assessments and the follow up of bioremediation techniques.
Particular importance is given to the understanding of theoretical concepts and their application to real situations.
  Table of contents (summary; under construction)
  General Introduction
Sources and fate of pollutants in terrestrial environments
Effects of pollutants on soil quality
Overview of standard methods for soil ecotoxicology testingGeneral introduction


  • Bioassays for regulatory purposes
  • Monitoring: microcosms, mesocosms & field tests
Soil bioremediation


  • Definition and history of bioremediation
  • Soil as habitat for microorganisms          
  • Principles of microbial degradation of pollutants
  • Soil bioremediation
  • Case studies of soil bioremediation
Phytoremediation


  • Importance of metals in plant growth
  • Plant diversity and metal immobilization
  • Phytoremediation
NOTE:
As part of your university education, you can legally dispose of the materials made available to you (pdf Power Points, videos, podcasts, etc.) protected by copyright. These materials are intended only to be used by students as part of their studies at the University of Liège. No other use or distribution is authorized, under penalty of constituting a violation of the law of June 30, 1994 relating to copyright. If the resources were to be freely accessible on the web, the person responsible would be exposed to legal proceedings.

Learning outcomes of the learning unit

At the end of this course, students should be able to:


  • discuss the concept of soil quality in relation to ecosystem services
  • define soil ecotoxicology and defend its use in soil protection
  • describe sources and fates of chemicals in terrestrial environments
  • characterize main soil properties and their importance in the fate of soil contaminants
  • explain bioavailability and mobility of pollutants
  • cite and explain main methods of soil ecotoxicology: individual bioassays and methods for the assessment of soil biota in situ
  • explain the role of microorganisms in the degradation of organic pollutants
  • perform quantitative assessment of risks posed by toxic pollutants to terrestrial ecosystems
  • describe the main processes used in soil bioremediation
  • understand the use and criteria for applying microbial activities in the processes of bioremediation
  • apply of course theory and techniques of analyses of case studies for approaching problems in environmental microbiology
  • evaluate of the relevance of a technique using microorganisms in environmental management
  • understand the use of plants for phytoremediation purposes
  • develop a critical view on the use of transgenic plants versus natural populations for phytoremediation
Learning objectives related to practicals:


  • acquire analytical technical and expertise skills for characterising and evaluating the effect of pollutants on soils
  • interpret and discuss the results of soil bioassays and studies with regard to ecological risk assessment
  • analyse and interpret element content data from plant tissues
  • develop synthesis, interpretation and writing skills

Prerequisite knowledge and skills

Students should be familiar with basic concepts of ecology, soil ecology, chemistry and ecotoxicology.

Planned learning activities and teaching methods

Lectures (power point support) are based on the principles of 'active lectures'. Presentations include questions, minute papers, case studies and class discussions. The link between theory and practice for the methods in soil ecotoxicology is established through the practicals/tutorials.  
The integration of lectures and various activities aims at a deep understanding of the topic during the quadrimester. Student's personal investment during lectures and activities will lead to a work reduction during the exam session and also leads to better knowledge retention on the longer term.

Mode of delivery (face to face, distance learning, hybrid learning)

Most of theoretical courses are lectures illustrated with power point presentations, videos, slide shows. The topics considered in the theoretical course will be illustrated and expanded in laboratory practicals, case studies prepared by students, data analyses.
 
Attendance of lectures and practicals (including the production of related reports) is mandatory. Should a student not fulfil these criteria, he/she will be considered "inadmissible" to the exam.
 
All useful resources for the course are available online via eCampus (power point illustrations, supporting documents, information, discussions,...). Note: downloading the documents is more efficient with a high-speed connexion. Computer rooms are available on campus.

Organisational adjustments related to the current health context

The course will be organized in the form of a 'flipped class', i.e. the students read the documents provided, then discussion sessions will allow the students to ask their questions and the teachers to check student's understanding of the course material.
 

Recommended or required readings

A work document for practicals will be provided to the students and it is strongly advised to read it before the practicals, for a better understanding of the work required and to prepare questions.
 
Lecture notes and reading material will be made available during the year. Power point presentations will be available online (pdf). For complementary information, reference books are available from ULiège library and the instructors:
 
Duarte A.C., Cachada A., Rocha-Santos T. (2017) Soil Pollution From Monitoring to Remediation (1st Edition), Academic Press, 312 p.

Assessment methods and criteria

Below you will find information on the evaluation methods planned for in-person and remote exams as well as those planned for hybrid sessions. Depending on how the health crisis evolves, the chosen method will be communicated to you no later than one month before the start of the exam session.

Any session :

- In-person

oral exam

- Remote

oral exam

- If evaluation in "hybrid"

preferred remote


Additional information:

Assessment consists in:






  • an appreciation of students' activities during practicals and tutorials. At the end of the practicals, each student will be asked to provide a written report (20%).
  • an oral exam (with written preparation), will be held during the session and will concern both the theoretical and practical courses (80%).
  REMINDER (see general examination rules): Cheating or plagiarism are severely sanctioned, leading to a score of 0/20 at the exam. Participation at practicals and the timely submission of reports is mandatory (students failing to comply with this rule may be declared inadmissible at the exam). Any delay in the submission of requested work results in a score of 0/20.

Work placement(s)

Organizational remarks

Contacts

Office : by appointment only    

Laboratoire d'écologie animale et d'écotoxicologie, Institut de Chimie, Bât. B6c, Allée du 6 août, 15, 4000 Liège, Belgique


  • Dr Célia Joaquim-Justo : Tél. 04/366.51.40 ; Fax 04/366.51.47 ; E-mail : celia.Joaquim-Justo@uliege.ac.be

Laboratoire d'Ecologie Végétale et Microbienne, Bât. B22, Chemin de la Vallée, 4, 4000 Liège, Belgique (3ème étage)


  • Prof. Monique Carnol : Tel. 04/3663845 (bureau) ; Tel. 04/3663817 (laboratoire), E-mail : m.carnol@uliege.ac.be                   
Génomique fonctionnelle et imagerie moléculaire végétale, Bât. B22, chemin de la Vallée 4, 4000 Liège, Belgique (2ème étage, local 2.12)


  •  Dr Marc Hanikenne : Tel. 04/3663844 (bureau) , E-mail : marc.hanikenne@uliege.be