Duration
25h Th, 15h Mon. WS
Number of credits
Lecturer
Coordinator
Language(s) of instruction
French language
Organisation and examination
Teaching in the first semester, review in January
Schedule
Units courses prerequisite and corequisite
Prerequisite or corequisite units are presented within each program
Learning unit contents
Partim XR
The different experimental steps that will lead to the image of a biomolecule using the technique of X-ray diffractionby monocrystal will be described. Three examples illustrating the relations structure-function will be approached, on the basis of research articles describing structural analysis of different proteins illustrating the following 3 families: - proteins controlling the DNA transcription, - serine proteases, - membrane proteins.
Partim CryoEM
We will discuss the steps required for the determination of a protein structure by the single particule cryo-electron microscopy technique. We will describe recent technological advances that have led to the very rapid emergence of this technique in structural biology and discuss its advantages and limitations. Some recent examples of structures illustrating the power of the technique will be described.
Partim NMR
The course deals with various aspects of structural biology for which the NMR provides essential information such as : Solution structure dynamic Interraction of all kinds Protein folding intrinsically disordered proteins
The different concepts of NMR and NMR techniques are seen according to the different aspects of structural biology considered.
Learning outcomes of the learning unit
Partim XR
This course gives to the future biochemists the bases necessary to understand the principles of determination of 3D structures by X-ray crystallography in order to enable them to make an objective use of the crystallographic models.
Partim CryoEM
The purpose of this section is to provide information to enable students to know the potential of the technique for determining biological structures for its integration into future research projects.
Partim NMR
Understand how NMR contributes to the understanding of proteins and their function.
Prerequisite knowledge and skills
Course of physics of 1st cycle. Basic knowledge in X-ray crystallography and knowledge of the basic principles of the structures of proteins.
Planned learning activities and teaching methods
This lecture will be given in a classical form, including the use of a PowerPoint presentation, a chalk and a black board.
Mode of delivery (face to face, distance learning, hybrid learning)
Organisational adjustments related to the current health context
The lessons will be given by videoconference. Classes will be recorded and made available to students.
The exam will consist of an oral presentation of a research article combining several techniques seen in the lectures. This presentation will be done as far as possible in pairs.
The articles will be communicated to the students at the end of the course.
Recommended or required readings
CD rom including Lectures powerpoint presentations, research articles, PDB files and tutorials will be available. Reference books : - "Crystallography made hook clear: With guide for users for macromolecular models." Scale Rhodos, Academic Press 2000 - "Introduction to Protein Structure" Carl Branden and John Tooze, Garland Publishing 1999 CD roms: - PS ² and Kinemage Supplement to "Introduction to Protein Structure" Carl Branden and John Tooze, Garland Publishing 1999
Assessment methods and criteria
Below you will find information on the evaluation methods planned for in-person and remote exams as well as those planned for hybrid sessions. Depending on how the health crisis evolves, the chosen method will be communicated to you no later than one month before the start of the exam session.
Any session :
- In-person
oral exam
- Remote
oral exam
- If evaluation in "hybrid"
preferred in-person
Additional information:
The course evaluation will be based on the choice of a scientific article in English.
The exam will be oral and will consist of a powerpoint presentation, followed by interactive questions.
Work placement(s)
Organizational remarks
Contacts
Paulette CHARLIER, PhD, Professeur
Frédéric Kerff, PhD, Chercheur qualifié FNRS
DAMBLON Christian, PhD, Professeur