Duration
20h Th
Number of credits
| Master in biomedicine (120 ECTS) | 2 crédits |
Lecturer
Language(s) of instruction
English language
Organisation and examination
Teaching in the second semester
Schedule
Units courses prerequisite and corequisite
Prerequisite or corequisite units are presented within each program
Learning unit contents
Quantitative cytometry: individual analysis of a large number of cells by two complementary approaches including the detection of rare cells of a great biological interest (cancer cells). 1) image cytometry: presentation on all optical microscopes, confocal, phase, fluorescence, interference, electronic, and morphomathematical analysis of the images acquired by these instruments: image segmentation, binary morphometry, the watershed transformation , Fourier analysis, cell identification, counting and measurements of morphometric parameters. 2) Flow cytometry: analysis multifluorescence and high-speed sorting of thousands of cells per second. Multicolor immunofluorescence, cell cycle, calcium flux.
Learning outcomes of the learning unit
functionnal principles of the different apparatus and methods
Prerequisite knowledge and skills
no prerequisite
Planned learning activities and teaching methods
oral présentation
Mode of delivery (face-to-face ; distance-learning)
face to face
Recommended or required readings
nothing
Assessment methods and criteria
oral exam with open book: any notes are authorized; discussion of understanding.
Work placement(s)
NULL
Organizational remarks
courses are given exclusively in French
Contacts
Prof. Roland Greimers E-mail: Roland.Greimers@chu.ulg.ac.be Tél: 04/366.24.12
Adaptation of teaching commitments following the COVID-19 pandemic for the May-June 2020 session
Teaching methods implemented : distance-learning
cursus is online as usual
Assessment subjects
all uploaded ppt and pdf are the cursus content
Assessment methods
Interview 10-15 minutes, via phone or Lifesize
Evaluation of the general understanding of technical concepts
Contacts
Professeur Roland Greimers E-mail: Roland.Greimers@chuliege.be Tél: 04/366.24.12
Adaptation of teaching commitments following the COVID-19 pandemic for the Aug-Sept 2020 session
Assessment subjects
the online notes and the courses seen are the subject to know
Assessment methods
All notes are authorized exactly as in face-to-face. The assessment is a 15-minute interview (oral exam) by phone or email of pure understanding, comparison of methods; quick question and answer without prior preparation.
Contacts
Roland.Greimers@chuliege.be 04 366 24 12
Items online
2020-Introduction
Copyright rules
microscopy and binary morphology
Copyright usual rules
special techniques-Voronoi-Fourier-watershed segmentation
special techniques-Voronoi-Fourier-watershed segmentation
Lesson 4-Flow cytometry -part 1
Lesson 4-Flow cytometry -part 1
spectral and mass cytometry
spectral and mass cytometry
Super high resolution microscopy
Super high resolution microscopy