Duration
8h Th, 20h Pr
Number of credits
| Master of Science (MSc) in Biomedical Engineering | 2 crédits | |||
| Bachelor in biomedicine | 2 crédits | |||
| Master in biology of organisms and ecology (120 ECTS) | 3 crédits |
Lecturer
Language(s) of instruction
French language
Organisation and examination
Teaching in the first semester, review in January
Schedule
Units courses prerequisite and corequisite
Prerequisite or corequisite units are presented within each program
Learning unit contents
The course is subdivided in the following way:
- cellular lines
- techniques of aseptie
- environment of the culture
- primary cultures
- techniques of observation in optical microscopy
- contaminations
- instability, variation and conservation
- measurement of viability and cytotoxicity
- three-dimensional culture
Learning outcomes of the learning unit
The philosophy of the course of culture of cells is to communicate to the students the basic techniques used for the culture of animal or human cells in vitro
The objective continued are:
- maintenance of the viable isolated cells or the functional units apart from the organism;
- application of good laboratory practices;
- handling, which constitutes the best training for the student.
Prerequisite knowledge and skills
Good knowledge in cellular and tissue biology
Planned learning activities and teaching methods
- Preparation of sterilized material and media
- Starting cells culture
- Congelation -decongelation
- Cells count
- Passaging
- Viability tests
- Observations (phase contrast microscopy)
Local: 3/62 - Institute of chemistry (B6c)
Mode of delivery (face-to-face ; distance-learning)
First semester Course: face-to-face TP: in L2 Laboratory
Recommended or required readings
Notes de travaux pratiques
Dias (slideshow) on line
Ouvrage de référence: Culture of animal cells: a manual of basic technique and specialized applications. R. Ian Freshney - 7th edition - Wiley Editions (available at the library of Sciences de la Vie - CHU)
Assessment methods and criteria
Theory: written examination in january
Work placement(s)
Organizational remarks
Labcoat mandatory Laboratory book
Contacts
Dr. Erik Maquoi & Dr. Julie Lecomte
Laboratoire de Biologie des Tumeurs et du Développement
Tour de Pathologie, B23 (+4), route 688
Avenue Hippocrate, 13
Sart - Tilman
B 4000 Liège
Tél: 04/366.25.31
E-mail: Erik.Maquoi@uliege.be / jlecomte@uliege.be
Adaptation of teaching commitments following the COVID-19 pandemic for the May-June 2020 session
Teaching methods implemented : distance-learning
Assessment subjects
Assessment methods
Contacts
Adaptation of teaching commitments following the COVID-19 pandemic for the Aug-Sept 2020 session
Assessment subjects
Theoritical and practical exercices.
Assessment methods
An examination will be available on eCampus (total duration: max 120 minutes).
Contacts
Dr. Erik Maquoi
Laboratoire de Biologie des Tumeurs et du Développement
Tour de Pathologie, B23 (+4), route 688
Avenue Hippocrate, 13
Sart - Tilman
B 4000 Liège
Tél: 04/366.25.31
E-mail: Erik.Maquoi@uliege.be