Genetic engineering

Duration

20h Th

Number of credits

Lecturer

Jacques Dommes

Language(s) of instruction

French language

Organisation and examination

Teaching in the first semester, review in January

Schedule

Schedule online

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

1. Manipulation of DNA fragments - Restriction enzymes - Enzymatic synthesis of DNA - Ligation of DNA fragments
- Dephosphorylation and phosphorylation of DNA - DNA labeling 2. Genetic engineering of Eubacteria - Plasmid vectors - Lambda phage vectors - Cosmids - BAC - Vectors for in vitro transcription - Expression vectors for the production of heterologous proteins in E. coli - Expression of recombinant proteins in other bacteria
3. Site-directed mutagenesis and genome editing - Random mutagenesis of a gene - Mutagenesis by DNA fragment exchange - Mutagenesis by PCR - Genome editing
4. Genetic engineering of yeasts and filamentous fungi - Introduction - Transformation methods in yeasts - Vectors for Saccharomyces cerevisiae - Promoters for S. cerevisiae - Secretion of the recombinant protein - Other yeasts and fungi - The two-hybrid system
5. Plant genetic engineering - Transformation via Agrobacterium tumefasciens and the Ti plasmid - Gene gun method - Selection and reporter genes - Chloroplast genome transformation - Viral vectors - Inverse genetics in plants - Examples
6. Production of recombinant proteins in animal cells - Methods of transfection - Insect cells - Mammalian cells
7. Transgenic animals and genetherapy - Vectors - Integration into the genome - Transgenic mice - Transgenesis in other mammals and in birds - Reproductive cloning in mammals - Transgenic fishes

Learning outcomes of the learning unit

· To get acquainted with the main methods used in genetic engineering
· To get informed of the potential of genetic engineering in the study of living organisms, as well as in the improvement of species for human use and for the production of recombinant proteins.

Prerequisite knowledge and skills

Molecular biology and genetics (courses of the bachelier in Biology).

Planned learning activities and teaching methods

Mode of delivery (face-to-face ; distance-learning)

Lectures during first period.

Recommended or required readings

Powerpoint presentations and lecture texts available through the eCampus system.

Assessment methods and criteria

Oral exam (first exam exam session, as well as second one).

Work placement(s)

Organizational remarks

Contacts

- Prof. J. Dommes (j.dommes@ulg.ac.be)

Adaptation of teaching commitments following the COVID-19 pandemic for the May-June 2020 session

Teaching methods implemented : distance-learning

Assessment subjects

Assessment methods

Contacts

Adaptation of teaching commitments following the COVID-19 pandemic for the Aug-Sept 2020 session

Assessment subjects

All topics seen during the lectures held during the first course period.

Assessment methods

Oral exam through Lifesize (connexion details will be communicated a few days before the exam).

Contacts

j.dommes@uliege.be

Items online