Duration
25h Pr, 15h Th
Number of credits
| Master of Science (MSc) in Biomedical Engineering | 4 crédits |
Lecturer
Coordinator
Language(s) of instruction
French language
Organisation and examination
Teaching in the second semester
Schedule
Units courses prerequisite and corequisite
Prerequisite or corequisite units are presented within each program
Learning unit contents
THEORETICAL PART
1- Basis of protein folding and major techniques used to investigate protein folding in vitro
2- In vivo protein folding
- Main techniques used to investigate protein folding in vivo
- Role of chaperones
3- Degradation of misfolded proteins (proteosome)
4- Protein misfolding and diseases
- Diseases associated with amyloid firbil formation
6- Functional amyloids
- Amyloids as functional coats for microorganisms
- Amyloids as orchester of the biosynthesis of melanin in mamalian tissue
- Amyloid fibrils as nanomaterial
7- Nanobodies: structure, properties and therapeutic applications
PRACTICAL PART
First the structural propertties and the stability of a serie of known proteins are investigated. Then, based on these data, the nature of three unknown samples is investigated. The techniques used include: absorbancy, fluorescence, circular dichroism and electronic microscopy.
Learning outcomes of the learning unit
A theoretical overview of techniques of used in studying the structure, stability and function of proteins is given. Applications include classical equiilibrium thermodynamics and spectroscopic methods including: UV-Vis spectroscopy, circular dichroism, fluorescence.
Prerequisite knowledge and skills
Planned learning activities and teaching methods
Mode of delivery (face-to-face ; distance-learning)
Recommended or required readings
Assessment methods and criteria
the valuation will be based on the presentation of the results of the practical work
Work placement(s)
Organizational remarks
The PowerPoint presentations of the theoretical course will be available on CD as well as the notes of practical works.
Contacts
Mireille Dumoulin
Research Associate F.R.S.-FNRS
Centre for Protein Engineering,
Laboratory of Enzymology and Protein Folding
Institut of Chemistry, B6
University of Liege
Sart Tilman,
B4000 LIEGE, Belgium
Tel: 0032 (0)4 366 3546
Fax: 0032 (0)4 366 3364
Adaptation of teaching commitments following the COVID-19 pandemic for the May-June 2020 session
Teaching methods implemented : distance-learning
Assessment subjects
Assessment methods
N.a.