Duration
15h Th
Number of credits
| Doctoral training in sciences (BBMC) | 3 crédits |
Lecturer
Coordinator
Language(s) of instruction
French language
Schedule
Units courses prerequisite and corequisite
Prerequisite or corequisite units are presented within each program
Learning unit contents
The course presents the different expression and production systems of recombinant proteins, including their purification, in Gram negative (E. coli) or positive (Bacillus, Streptomyces and Lactococcus) bacteria as well as the in vitro expression systems.
The attention is focused on the main protein characteristics to consider before choosing an expression system.
the course is given in french and notes are available at the exact cost price
Learning outcomes of the learning unit
At the end of the lectures, the student will be able to pay attention to the important characterictics of the target protein and to choose the optimal expression and production system as well as the best suitable host for getting a good production.
Prerequisite knowledge and skills
Knowledges in molecular biology and genetic engineering are necessary to follow this course.
Planned learning activities and teaching methods
Teaching with powerpoint slides
Mode of delivery (face-to-face ; distance-learning)
Face-to-face
Recommended or required readings
Assessment methods and criteria
The evaluation consists in discussing the proposed expression system for a protein indicated by the teacher at the end of the course and is followed by an oral discussion.
Work placement(s)
Organizational remarks
Usually, five lectures are given to present the content of the course.
The students are invited to contact me to know the proposed schedule in agreement with them.
Contacts
cduez@ulg.ac.be
Tél.: 04 366 33 77
Institute of chemistry, B6a, office1.62