LABO0433-2

Duration

15h Th, 5h Pr

Number of credits

	Master in biomedicine (120 ECTS)	2 crédits
	Master in biomedicine (60 ECTS)	2 crédits

Lecturer

Roland Greimers

Language(s) of instruction

French language

Organisation and examination

Teaching in the first semester, review in January

Schedule

Schedule online

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

Quantitative cytometry: individual analysis of a large number of cells by two complementary approaches including the detection of rare cells of a great biological interest (cancer cells). 1) image cytometry: presentation on all optical microscopes, confocal, phase, fluorescence, interference, electronic, and morphomathematical analysis of the images acquired by these instruments: image segmentation, binary morphometry, the watershed transformation , Fourier analysis, cell identification, counting and measurements of morphometric parameters. 2) Flow cytometry: analysis multifluorescence and high-speed sorting of thousands of cells per second. Multicolor immunofluorescence, cell cycle, calcium flux.

Learning outcomes of the learning unit

functionnal principles of the different apparatus and methods

Prerequisite knowledge and skills

no prerequisite

Planned learning activities and teaching methods

oral présentation

Mode of delivery (face-to-face ; distance-learning)

face to face

Assessment methods and criteria

oral exam with open book: any notes are authorized; discussion of understanding.

Work placement(s)

NULL

Organizational remarks