Genetic engineering

Duration

20h Th

Number of credits

Lecturer

Jacques Dommes

Language(s) of instruction

French language

Organisation and examination

Teaching in the first semester, review in January

Schedule

Schedule online

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

1. Introduction - Definitions - Basics of molecular biology - Genome sizes
2. Manipulation of DNA fragments - Restriction enzymes - Enzymatic synthesis of DNA - Chemical systhesis of DNA - Ligation of DNA fragments - DNA labeling - Separation of DNA fragments
3. Genetic engineering of Prokaryotes - Plasmid vectors - Lambda phage vectors - Cosmids - Vectors for in vitro transcription - Expression vectors for the production of heterologous proteins in E. coli - Expression of recombinant proteins in other bacteria
4. Construction and screening of DNA libraries
- Number of clones in a DNA library - Construction of a DNA library - Screening of a DNA library by hybridisation - Screening of a DNA library constructed in an expression vector
5. Site-directed mutagenesis - Random mutagenesis of a gene - Mutagenesis by DNA fragment exchange - Primer elongation - Mutagenesis by PCR - Genome editing
6. Specific application of PCR - Contaminations in PCR - PCR-ELISA - Quantitative PCR and RT-PCR - In situ PCR - Cloning PCR products - Asymetric PCR - Nested PCR
7. Genetic engineering of yeasts and filamentous fungi - Introduction - Transformation methods in yeasts - Vectors for Saccharomyces cerevisiae - Promoters for S. cerevisiae - Secretion of the recombinant protein - Other yeasts and fungi - The two-hybrid system

Learning outcomes of the learning unit

· To get acquainted with the main methods used in genetic engineering
· To get informed of the potential of genetic engineering in the study of living organisms, as well as in the improvement of species for human use and for the production of recombinant proteins.

Prerequisite knowledge and skills

Molecular biology and genetics (courses of the bachelier in Biology).

Planned learning activities and teaching methods

Mode of delivery (face-to-face ; distance-learning)

Lectures during first period.

Recommended or required readings

Powerpoint presentations and lecture texts available through the eCampus system.

Assessment methods and criteria

Oral exam (first exam exam session, as well as second one).

Work placement(s)

Organizational remarks

Contacts

- Prof. J. Dommes (j.dommes@ulg.ac.be)

Items online