2018-2019 / BIOL0029-1

Practical genomics

Duration

10h Th, 30h Mon. WS

Number of credits

 Master in bio-informatics and modelling (120 ECTS)4 crédits 

Lecturer

Denis Baurain, Marc Hanikenne

Coordinator

Denis Baurain

Language(s) of instruction

English language

Organisation and examination

Teaching in the second semester

Schedule

Schedule online

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

High throughput sequencing technologies have revolutionized Biology. Analyzing the data they generate requires the implementation of bioinformatic tools and the use of high-performance computers.
This course provides a practical approach to illustrate concepts and tools for analyzing genomic data. It is composed of different modules:

  • Module 1. Assembling a genome
  • Module 2. Introduction to the use of high performance computers (HPC)
  • Module 3. Introduction to Markdown reporting
  • Module 4. RNA-Seq data analysis
  • Module 5. Analysis of genomic sequence variants
  • Module 6. Analysis of metagenetic data
  • Module 7. Phylogenetic analyses
 

Learning outcomes of the learning unit

By the end of the course, students will have acquired basic practical knowledge for the analysis of high throughput sequencing data. The course involves intense use of the command line, interaction with a computing cluster, and the use of popular analysis pipelines.

Prerequisite knowledge and skills

Genomics (GENE0003-1) and bioinformatics (BIOL0008-1) concepts acquired at the end of block 1 of the Master BBMC or BIM are required to take the course. Similarly, the practical skills of course INFO0953-1 (Scripting interfaces for biological software and databases) are also required.

Planned learning activities and teaching methods

Each module starts with a brief theoretical introduction, before moving to computer practicals in a dedicated computer room.

Mode of delivery (face-to-face ; distance-learning)

Modules 1 to 3 are organized face-to-face. For modules 4 to 7, students work independently and a meeting of 1 hour per week is scheduled with the teachers to discuss the progress of the work and problems encountered.

Recommended or required readings

Course notes, PowerPoint slides and datasets to be analyzed are made available to students through myULiège.

Assessment methods and criteria

The analyses conducted in modules 4 to 7 are the subject of a written report summarizing the objectives, the analyses implemented and the results, using Markdown technologies.

Work placement(s)

Organizational remarks

The course is taught in English. The material is written in English.

Contacts

Prof. Denis Baurain Institut de Botanique B22 (P70) denis.baurain@uliege.be 
Dr. Marc Hanikenne Institut de Botanique B22 (P70) marc.hanikenne@uliege.be 
Assistant: Dr. Damien Sirjacobs Institut de Botanique B22 (P70) 04/366.38.54 D.Sirjacobs@uliege.be