Duration
15h Th, 5h Mon. WS
Number of credits
Lecturer
Language(s) of instruction
French language
Organisation and examination
Teaching in the first semester, review in January
Schedule
Units courses prerequisite and corequisite
Prerequisite or corequisite units are presented within each program
Learning unit contents
The different experimental steps that will lead to the image of a biomolecule using the technique of X-ray diffractionby monocrystal will be described. Three examples illustrating the relations structure-function will be approached, on the basis of research articles describing structural analysis of different proteins illustrating the following 3 families: - proteins controlling the DNA transcription, - serine proteases, - membrane proteins.
Learning outcomes of the learning unit
This course gives to the future biochemists the bases necessary to understand the principles of determination of 3D structures by X-ray crystallography in order to enable them to make an objective use of the crystallographic models.
Prerequisite knowledge and skills
Course of physics of 1st cycle. Basic knowledge in X-ray crystallography and knowledge of the basic principles of the structures of proteins.
Planned learning activities and teaching methods
This lecture will be given in a classical form, including the use of a PowerPoint presentation, a chalk and a black board.
Mode of delivery (face-to-face ; distance-learning)
Recommended or required readings
CD rom including Lectures powerpoint presentations, research articles, PDB files and tutorials will be available. Reference books : - "Crystallography made hook clear: With guide for users for macromolecular models." Scale Rhodos, Academic Press 2000 - "Introduction to Protein Structure" Carl Branden and John Tooze, Garland Publishing 1999 CD roms: - PS ² and Kinemage Supplement to "Introduction to Protein Structure" Carl Branden and John Tooze, Garland Publishing 1999
Assessment methods and criteria
A written or oral exam will be organized.
Work placement(s)
Organizational remarks
Contacts
Paulette CHARLIER Institut de Physique, Bât. B5a - 1/41 Sart-Tilman, 4000 Liège Call 00 32 (0)4/366.36.19 Fax 00 32 (0)4/366.49.54 E-mail : Paulette.Charlier@ulg.ac.be