2017-2018 / BIOC0719-2

Enzymology

Duration

15h Th, 25h Pr

Number of credits

 Master in bio-informatics and modelling (120 ECTS)5 crédits 

Lecturer

André Matagne

Language(s) of instruction

French language

Organisation and examination

Teaching in the first semester, review in January

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

Chapter 1: General properties of enzymes. Chapter 2: Steady-state kinetics. Chapter 3: Enzyme inhibition. Chapter 4: Effect of pH, temperature, viscosity and other experimental factors. Chapter 5: Reactions of two substrates. Chapter 6: Transient-state kinetics. Chapter 7: Cooperativity and allosteric interactions. Chapter 8: Enzyme inactivators. Chapter 9: Enzyme cofactors. Chapter 10: Mechanism of chymotrypsin. Chapter 11: Basic catalytic principles. Chapter 12: Regulation of catalytic activity.

Learning outcomes of the learning unit

The aim of the course is to make students familiar with both enzyme mechanisms and fundamentals of enzyme kinetics.

Prerequisite knowledge and skills

Courses on "Introduction to Enzymology" (BAC3 Biology) or "Biochemistry" (BAC3 Chemistry), which include an introduction to enzyme catalysis (chapters 1-3).

Planned learning activities and teaching methods

Most lectures are given using the blackboard and (hopefuly) white chalk.

Mode of delivery (face-to-face ; distance-learning)

face-to-face only

Recommended or required readings

Lecture notes will be available following the lectures.
Reference book: A. Cornish-Bowden, Fundamentals of enzyme kinetics, fourth edition, Wiley-Blackwell, 2012

Assessment methods and criteria

Oral exam

Work placement(s)

Non applicable

Organizational remarks

Same schedule as for BIOC0719-1, planned as decided by the professor and students

Contacts

Professor André Matagne, PhD, Enzymology and Protein Folding, Centre for Protein Engineering, Life Sciences Department, Institut de Chimie B6c (room 3/1), Allée de la Chimie, 3, University of Liège, B4000 Liège (Sart-Tilman), Tel.: +32 (0)4 3663419, Fax: +32 (0)4 3663396, Email: amatagne@ulg.ac.be