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| BIOL0014-1 | Dynamic molecular imaging
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| Duration : | 20h Th |
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| Number of credits : |
| Master in Biochemistry and Molecular and Cell Biology, Research Focus, 1st year | | 2 |
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| Master in Biochemistry and Molecular and Cell Biology, Teaching Focus, 1st year | | 2 |
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| Master en biochimie et biologie moléculaire et cellulaire, à finalité spécialisée en bio-industrie, 1st year | | 2 |
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| Master in Biochemistry and Molecular and Cell Biology, Professional Focus in
Industrial Biochemistry, 1st year | | 2 |
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| Master en biochimie et biologie moléculaire et cellulaire, à finalité spécialisée en bioinformatique et modélisation, 1st year | | 2 |
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| Lecturer : | Patrick Motte |
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Language(s) of instruction :
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| French language |
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Organisation and examination :
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| Teaching in the second semester |
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Course contents :
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| The course will be focus on dynamic and molecular imaging in living cells:
loss of fluorescence approaches (FRAP, iFRAP, FLIP) ; protein-protein interactions (FRET, BiFC) ; Fluorescence Correlation spectroscopy (FCS) and Fluorescence Lifetime Imaging. |
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Learning outcomes of the course :
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| To visualise (macro)molecules and macromolecular structures and their interactions over time and space is essential to the understanding of the physiology of biological systems: from the basic organisation of a cell to the complex development of organisms. Molecular imaging today presents many possibilities - reaching the level of the nanometre and the millisecond - and it is used in the study of the dynamics of certain processes of great interest in living and functioning cells in various physiological situations, and under stress. Considerable advances in the area of cellular biology are continually being helped by technological advances. These include confocality, multiphoton, high resolution and high sensitivity detectors, etc. |
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Prerequisites and co-requisites/ Recommended optional programme components :
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| Knowledge in cellular and molecular biology and in methods of molecular biology and genetic engineering. |
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Planned learning activities and teaching methods :
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| Practicals are integrated to the course 'Stages ou travaux pratiques intégrés (y compris séminaires)'. |
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Mode of delivery (face-to-face ; distance-learning) :
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| Lectures are given during the second quadimester to students of Master1 BBMC who have chosen the module in 'Physiology & Developmental Biology'. Courses are ex cathedra lectures and tutoring of personal work. |
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Recommended or required readings :
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| Illustrations and copy of powerpoint slides. Recommended textbook: Molecular Cell Biology 7th Ed, H. Lodish et al. 2013, W.H. Freeman and Company. |
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Assessment methods and criteria :
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| The evaluation will include a personal project on a topic proposed by the student or chosen among a list of suggestions. This project could involve different courses of the 'Physiology - Developmental Biology' module and contribute to a common evaluation. The student will be exempted from the chapters covered by his personal project for the exam (oral, according to student preferences). |
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Work placement(s) :
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| 5-week training periods are proposed in the course 'Stages ou travaux pratiques intégrés (y compris séminaires)' |
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Organizational remarks :
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Contacts :
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| Patrick Motte, patrick.motte@ulg.ac.be, 04 3663810 |
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