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| SDOC0039-1 | Christallographie appliquée aux biomolécules
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| Duration : | 20h Th, 15h Pr |
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| Number of credits : |
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| Lecturer : | Paulette Charlier |
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Language(s) of instruction :
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| French language |
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Course contents :
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| The aim of this course is to make the description of the X-ray (Neutron) Crystallography techniques used to investigate the structure of the biological macromolecules.
Theoretical part: 1- Overview of the crystallographic technique, 2- High resolution structure, 3- Neutron diffraction, 4- Laue diffraction; 5-Macromolecular assemblies, 6- Viral capsides and ribosomal particles structures
Practical part: PYMOL tutorials for protein structures visualization and analysis.
Solving of a protein structure in silico on the basis of X-ray diffraction data. |
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Learning outcomes of the course :
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| This course gives the bases necessary to understand the principles of determination of 3D protein structure by X-ray crystallography in order to enable to make an objective use of the models. It gives an outline of the capacities and limits of the various crystallographic techniques. |
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Prerequisites and co-requisites/ Recommended optional programme components :
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| Course of physics of 1st cycle. Basic knowledge in X-ray crystallography. Good knowledge of the basic principles of the structures of proteins. Biophysical course. |
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Planned learning activities and teaching methods :
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| Tutorials dedicated to the Pymol software use: obseravtion and analysis of macromolecules structures. Use of PDB database. Solving of a protein structure in silico on the basis of X-ray diffraction data. |
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Mode of delivery (face-to-face ; distance-learning) :
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| To be decided in concertation with the different lecturers. |
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Recommended or required readings :
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| The PowerPoint presentations of the theoretical course will be available on CD as well as the notes of practical works.
Books recommended:
- "Crystallography made hook clear: With guide for users for macromolecular models."
Scale Rhodos, Academic Press 2000
- "Introduction to Protein Structure" Carl Branden and John Tooze, Garland Publishing 1999
CD roms:
- PS ² and Kinemage Supplement to "Introduction to Protein Structure" Carl Branden and
John Tooze, Garland Publishing 1999 |
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Assessment methods and criteria :
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| To be decided in concertation with the different lecturers. Most probably on the basis of a research article. |
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Contacts :
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| Paulette Charlier, PhD, Chargé de CoursCristallographie des macromolécules biologiques, Centre d'Ingénierie des Protéines, Département des Sciences de la Vie, Université de Liège, Institut de Physique, B5a, B-4000 Liège, Belgium
Tel: +32 (0)4 366 36 19
Fax: +32 (0)4 366 49 54
E-mail : Paulette.Charlier@ulg.ac.be
http://www.cip.ulg.ac.be/ |
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