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| CHIM9218-1 | Biospectroscopy and nuclear magnetic resonance applied to biology
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| Duration : | 20h Th, 15h Mon. WS |
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| Number of credits : |
| Master in Biochemistry and Molecular and Cell Biology, Research Focus, 1st year | | First semester | | 3 |
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| Master in Biochemistry and Molecular and Cell Biology, Teaching Focus, 1st year | | First semester | | 3 |
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| Master en biochimie et biologie moléculaire et cellulaire, à finalité spécialisée en bio-industrie, 1st year | | First semester | | 3 |
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| Master in Biochemistry and Molecular and Cell Biology, Professional Focus in
Industrial Biochemistry, 1st year | | First semester | | 3 |
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| Master en biochimie et biologie moléculaire et cellulaire, à finalité spécialisée en bioinformatique et modélisation, 1st year | | First semester | | 3 |
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| Master in Biological Sciences | | First semester | | 3 |
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| Lecturer : | Christian Damblon |
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Language(s) of instruction :
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| French language |
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Course contents :
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| The course introduces different spectroscopies used in biochemistry.
Optical spectroscopies presented will be : UV-Vis Absorption, linear and circular dichroïsm, fluorescence, vibration spectroscopy and surface plasmon resonance.
for each method, theoretical principles, technical aspects and applications will be focused on proteins, nucleic acids and their ligand interactions.
NMR spectroscopy will be presented starting from simple experiments based on proton detection leading to more elaborate experiment dedicated to structural biology that involve carbon and nitrogen. Theoretical principles and technical aspects will be presented. Analytical exercises will be used to illustrate all the spectroscopies presented in the course |
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Learning outcomes of the course :
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| Increase the qualifications of the student concerning the spectroscopic methods available for thestudy of the conformational dynamics of proteins, nucleic acids and their interactions with small ligands. |
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Prerequisites and co-requisites/ Recommended optional programme components :
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| Chemistry and functional properties of biological macromlecules |
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Mode of delivery (face-to-face ; distance-learning) :
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| The lecture will be given as a classical form (as interactive as possible) |
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Recommended or required readings :
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| Lecture slides will be available (ppt format).
reference books :
- Biophysical Chemistry, Cantor and Schimmel
- Principles of Physical Chemistry, Van Holde et al.
- Biological Spectroscopy, Campbell and Dwek
- Physical Biochemistry, Sheehan
- An NMR primer for life scientists, Henry Rattle |
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Assessment methods and criteria :
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| The assessment will be made by a written examination |
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Contacts :
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| Christian Damblon, Professeur
Office : 3/71, Chemistry Institute, Sart-Tilman (B6c) 4000-Liège 1
Belgium
Call : 00 32 (0)4/3663788
Fax : 00 32 (0)4/3664577
e-mail : c.damblon@ulg.ac.be(P.Colson@ulg.ac.be) |
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