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| CHIM0254-1 | Biological analytical chemistry
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| Duration : | 20h Th, 40h Pr |
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| Credits/ECTS : |
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| Holder(s) : | Gauthier Eppe, Valérie Gabelica |
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| Language : | French language |
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| Course contents : | The course is divided into two distinct parts. In the first, the balanced reactions (acid-bases, redox, complexation, precipitation) are treated in terms of exchange of particles between donors and acceptors, thus unifying the concepts used in the study of the various reactions in aqueous solution. The next step consists in establishing a relationship between the variations of the conditional constants (acidity constants, formation constants of complex, normal potentials, exchange and partition constants) and the conditions of the medium (pH, ionic strength, complexing agent). These concepts being acquired, the implementation and the quantitative application of these various reactions with the purpose of analysis (titrimetry and gravimetry, primarily) are considered while insisting on the precision and exactitude which one should expect. The second part of the course treats on the conditioning of a sample which often constitutes an essential step for the analysis of its components: the isolation or separation of unwanted elements by Liquid-liquid extraction and chromatographic processes ( LLC, LSC, ion exchange and gel permeation) are then fully considered . |
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| Course objective : | To understand, interpret and apply the various reaction steps of an analysis protocol in order to obtain the best performances of exactitude and precision are the top priority of the course. This implies a perfect knowledge of the chemical reactions which it involves. |
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| Prerequisites : | The general chemistry course of the first year |
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| Workshops : | See below |
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| Organization : | Theoretical course: The lessons (1h45) begin the first Tuesday of the first semester at 10h45 and are given in the room R30 (Chemistry Building B6d - TP). The course uses multi-media tools: presentation on computer. An interruption of 5 to 10 minutes is provided at about the middle of each lesson.
Laboratory works: they begin first Wednesday of the first semester of the year (rooms R4 and R10, Chemistry building B6d -TP). They start at 11h until 18h (with an interruption for lunch between 13h and 14h) . The purpose is primarily to initiate the students with the specific requirements of the chemical analysis: do not lose anything, do not contaminate, great care, cleanliness and method, strict respect of the experimental protocols... The reliability, precision and exactitude of an analysis result depend, of course, on the choice of the method but also on the dexterity and competence of the experimentalist. |
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| Written notes : | The notes as well as the handbook of laboratory work are available at the "Cercle des Etudiants en Biologie". Exercises, simulation programs, practical information are distributed during the course. No particular material (except the apron) is necessary for the laboratories. The use of a handheld scientific calculator (programmable) is allowed, even advised for the solution of the exercises. As supplementary information, the consultation of the following work is highly recommended: - FUNDAMENTALS OF ANALYTICAL CHEMISTRY, 7th edition, Douglas A. Skoog, Donald Mr. West, F James Holler, Harcourt Publishers College, 1997 |
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| Assessment : | Practical laboratory work: the evaluation is made on the exactitude of the returned analysis results as well as on the theoretical understanding of the proposed protocols via two interrogations The final practical works score is calculated on the basis of 4 experiments + 2 interrogations.
Examination: The examination IS A WRITTEN exam. It involves exercises, theoretical questions directly from the course and applications: interpretation or layout of titration curves, calculations of conditional constants, chromatograms... The examination relies on the entirety of the course and includes 3 major parts: pHmetry, complexometry and separation methods . One can realize the importance allotted to practical works since, together with the laboratory interrogations, they count for 33.3% of the final score. |
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