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| BIOL2022-1 | Genetic engineering
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| Duration : | 12h Th |
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| Credits/ECTS : |
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| Holder(s) : | Richard Kettmann |
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| Language : | French language |
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| Course contents : | Theory :
- Bacteriophages : their use to construct cDNA expression librairies.
- Expression of a gene of interest using recombinant plasmids or viruses (baculovirus, retrovirus, vaccinia virus,...);
- Transgenic animals, Gene therapy;
- siRNAs and potential applications. |
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| Course objective : | To present the strategy leading to the cloning, the identification and the expression of a gene of particular interest with special emphasis on animal cells.
After completing the course the student is expected to
be able to propose the cloning of a gene in different expression systems |
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| Prerequisites : | -- KT204 - Molecular biology (2nd Part)
-- KT202 - Molecular biology (1st Part) |
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| Organization : | Lectures : 12h |
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| Written notes : | - Molecular Biotechnology - Principles and applications of recombinant DNA - B.R. GLICK and J.J. PASTERNAK - ASM Press, Washington DC, 1998, second edition.
- Principles of gene manipulation -S.B.Primerose, R.M.Twyman and R.W.Old-Blackwell Science-2001-sixth edition
- Recent reviews or articles
- Principles of gene manipulation. S.B. Primerose, R.M. Twyman and R.W. Old. Blackwell Sciences Sixth Edition 2001 |
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| Assessment : | Written examination (100%) |
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| Contacts : | Kettmann, Richard ( Directeur de recherches FNRS - chef de service)
Biologie cellulaire et moléculaire
081 62 21 56
kettmann.r@fsagx.ac.be |
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