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| BIOL2017-1 | Biological chemistry, 1st part
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| Duration : | 16h Th, 8h Pr |
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| Credits/ECTS : |
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| Holder(s) : | Bernard Wathelet |
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| Language : | French language |
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| Course contents : | Peptides and proteins : analysis and sequences, conformation, peptidic synthesis, interactions, purification Lipids : structure, analysis, alterations, transformations Physical and chemical properties Structure activitiy of proteins. Biomolecules analysis and purification methods. Precipitation, Partition, Ion exchange, Chromatography, Electrophoresis * Courses BIOL2017-1 et BIOL2017-2 are a whole |
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| Course objective : | To draw molecular and structural properties of biomolecules. Present analysis and purification methods for biomolecules After completing the course the student is expected to Understand the different principles of bio-molecules separation and be able to choose the right principle in function of the molecules to separate Understand the ionization phenomenon and predict the possible effect on association, biological activity. Be able to achieve a chromatographic separation (practical work) |
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| Prerequisites : | CHIM9237-1 - General chemistry CHIM9240-1 - Organic chemistry (first part) CHIM9239-1 - Biological molecules chemistry |
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| Workshops : | Extraction and purification of proteins by gel filtration. Determination of DM, nitrogen and essential amino acids in food. Quantitation of proteins by Folin method. Ion exchange chromatography. |
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| Organization : | Lectures : 16h Practical Works : 8h |
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| Written notes : | Lecture-notes |
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| Assessment : | Oral examination (90%) Group work (10%) |
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| Contacts : | Wathelet, Bernard (Chargé de cours) Université de Liège Gembloux Agro-Bio Tech Chimie biologique industrielle +32 81 62 22 25 bwathelet@ulg.ac.be |
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