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| BIOL0806-1 | Cell Biology and visualization methods
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| Duration : | 25h Th, 25h Pr |
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| Credits/ECTS : |
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| Holder(s) : | Patrick Motte, Marc Thiry |
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| Language : | French language |
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| Course contents : | 1. Molecular and dynamic imagery (P. Motte)
Part 1. Imaging tools
· Fluorescence, epifluorescence
· Confocality, multiphoton, detectors, deconvolution
Part 2. Dynamic molecular localisation
· From genes to proteins
· AFPs ; approaches to loss of fluorescence (FRAP, iFRAP, FLIP) ; protein-protein interactions (FRET, BiFC) ; Fluorescence Correlation spectroscopy (FCS) and Fluorescence Lifetime Imaging
2. Ultrastructural cytochemistry (M. Thiry)
· Introduction : transmission electron microscopy
· Different cytochemical methods (DNA : the Feulgen reaction, osmium-amine reaction; RNA : Brachet test, Bernhard's staining of EDTA; Acidic and basic proteins; Polysaccharides : PAS, TCH)
· Gold-complexes: lectin-gold, enzyme-gold, ..
· High resolution autoradiography
· Immunocytological methods and cryo-immunocytology
· Techniques of molecular biology adapted in ME : Hybridation, the tunnel method, la nick-translation, transcription in situ |
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| Course objective : | To visualise (macro)molecules and macromolecular structures and their interactions over time and space is essential to the understanding of the physiology of biological systems: from the basic organisation of a cell to the complex development of organisms. Molecular imagery today presents many possibilities - reaching the level of the nanometre and the millisecond - and it is used in the study of the dynamics of certain processes of great interest in living and functioning cells in various physiological situations, and under stress. Considerable advances in the area of cellular biology are continually being helped by technological advances. These include confocality, multiphoton, high resolution and high sensitivity detectors, cryomicroscopy, electron microscope tomography, deconvolution, etc. The object of the course is to acquaint students with these imaging tools briefly and to continue by examining applications of imaging techniques in the study of cells, and in the area of developmental biology. |
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| Prerequisites : | Cellular physiology and basic concepts of microscopy. |
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| Workshops : | To define. |
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| Organization : | Monday : course - Practical work
Tuesday : course - Practical work
Wednesday : course - Practical work
Thursday : preparation/discussion
Friday : article presentation |
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| Written notes : | Copies of slide shows (Power Point) used in class will be available. 3 students must sign up in order for the course to meet. |
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| Assessment : | The examination will consist in the presentation of a scientific article. The modalities of examination will be arranged in consultation with students. |
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| Contacts : | Prof. Patrick MOTTE, phone: 043663810 Email: patrick.motte@ulg.ac.be
Prof. Marc THIRY, phone: 043665169 Email : mthiry@ulg.ac.be |
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