 | |
| BIOC0205-4 | Macromolecular biochemistry
 |
|
| Duration : | 4h Vis. Ind. Pl. , 20h Th, 20h Pr |
|
| Credits/ECTS : |
|
|
| Holder(s) : | Moreno Galleni |
|
| Language : | Langue française |
|
| Course contents : | - Non covalent interactions
Hydrogen bound
Van der Waals interactions
Electrostatic interactions
Hydrophobic interactions
- Chemical properties of amino acids
- Introduction to the folding of protein
Chaperone
- Stabilisation and denaturation of proteins by physical and chemical methods
- Protein purification
Extraction and fractionation
puriifcation by chromatography
Analysis of protein sby electrophoresis
Determination of the protein concentration
Analysis of sample purity
-Introduction to the proteome analysis |
|
| Course objective : | The fisrt part of the lectures will describe the factor influencing the structure and the properties of proteins. The second part will be devoted to the different chromatography techniques used for the puriifcation of proteins. |
|
| Prerequisites : | Course of general biochemistry
Course of biophysic and general chemistry |
|
| Workshops : | 4 days of practical lectures deveoted to the purification and characterization of paravalbumine from pike
The laboratories will done at the following place:
bâtiment B6C- Institut de Chimie - Licence et Recherche - 2éme étage |
|
| Organization : | 10 lectures of 2 hours
4 days of practical lectures deveoted to the purification and characterization of paravalbumine from pike |
|
| Written notes : | Syllabus + Power point presentation |
|
| Assessment : | Oral exam |
|
| Contacts : | Theoretical lectures :
Galleni Moreno, Local 3/7, Institut de Chimie , B6. Sart Tilman
Tel:04/3663549 - Fax:04/3663364 - Email:mgalleni@ulg.ac.be
Practical lectures:
Dr. Feller Georges, Chef de Travaux
Institut de chimie B6 Sart Tilman
Tel 04/3663343 Fax 04/3663364 email:gfeller@ulg.ac.be
Berlemont Renaud, Assistant
local 1/34, institut de Chimie, B6a. Sart Tilman
Tel: 04/3663348- Fax: 04/3663396 - Email:rberlemont@student.ulg.ac.be |
|
