SBIM2029-1

Duration

40h Th, 10h Pr, 20h AUTR

Number of credits

	Master in biomedicine (120 ECTS)	5 crédits

Lecturer

François Boemer, Sébastien Bontems, Philippe Calmant, Benaïssa Elmoualij, Marie-Pierre Hayette, Cécile Meex

Language(s) of instruction

French language

Organisation and examination

Teaching in the first semester, review in January

Schedule

Schedule online

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

COORDINATOR : Hayette Marie-Pierre 25h Th, 25h Pr

Teachers : François Boemer, Philippe Calmant, Benaissa Elmoualij, Rosalie Sacheli, Sébastien Bontems, Cécile Meex

The module will include 4 modules provided by 4 groups of different teachers.

Quality assurance in laboratories - Philippe CALMANT - 2H Th

Content: Concept of quality assurance ; normative systems, accreditation bodies and audit procedures; documentation; quality manual and standardized procedures; quality coordinator's responsibility; validation of techniques and quality control; internal audits; corrective and preventive actions; complaint and claim management ; specific issues related to laboratories in clinical Biology.

Learning achievement: The course provides students with information about the implementation of a quality system in analysis laboratories, its management and development. It allows to get acquainted with the ISO 17025 standards, with a general scope for the test laboratories, and with the new ISO 15189 standards, applicable to biomedical laboratories.

Kind of teaching: face-to-face and distance learning - Written exam

Immunoassays - Benaïssa Elmoualij - 5H Th + 15H Pr

Learning achievement: The course firstly aims at introducing the immuno-detection tools. The basic tools will be antibodies. We will introduce the principles of the techniques used in immunological engineering. We will approach the reasons of the choice between various strategies used to produce antibodies and other tools as an alternative. The student will be led to understand the common applications of immunological engineering and to be able to propose the major elements of an experimental process that aims at solving a problem related to immuno-detection.

Kind of teaching: The exercises aim at illustrating the course by simulating problems related to immunological engineering met in research and development. Practical exercises will illustrate some key achievements in the field chosen in various scope of application: case of aptamers. During practical works, students will be made aware of the notion of the supports of immuno-detection and will carry out different experiments of immuno-detection by the development of ELISA and western blot.

GENERAL OVERVIEW AND STRUCTURE OF LESSONS

I- Introduction to immunological techniques
I-1. Introduction

I-2. Antibodies

I-3. Antigens / immunogens

I-4. Antigen-antibody reaction

II- Tools of immunoassays

II-1. Antibodies / antigens

II-2. Immunassays : « Use and limits »

II-3. Hybrid systems of immunoassays: aptamers, aptagenes, affinity peptides, etc.

II-4. Markers and performance measure of immunoassays
III- Scientific watch and development in techniques of immunoassays

Diagnosis of rare diseases: complementarity between metabolomics and genomics - François Boemer - 5H Th

Content: The course deals with the perspectives of genetic diagnosis of rare diseases thanks to metabolomics and genomics and their principles in diagnosis.

Kind of teaching: face-to-face, ex-cathedra classes, written exam

Clinical microbiology - Sébastien Bontems, Cécile Meex, Rosalie Sacheli - 5H Th + 10H Pr

Content :

Theoretical part

The theoretical lessons are held in 5 hours:

2 hours given by Cécile Meex that will introduce the flow of a microbiological sample within dispatching fields, bacteriology and serology and will briefly introduce the various analyses proposed by the different fields.
2 hours given by Sebastien Bontems that will introduce the flow of a sample and the various analyses within the field of molecular biology and the AIDS-Reference laboratory, as well as the general principles of quality assurance in microbiology.
1 hour given by Rosalie Sacheli that will explain the role of the national centres of reference within the microbiology laboratory and their various tasks at the national level (transfer of data to Sciensano, annual epidemiological report writing, etc.)

Practical part

Observation of one field of the clinical microbiology laboratory at the CHU in Liège by group (bacteriology, molecular biology, national centres of reference, serology, etc.).

To identify the flow of work by identifying the facilities, to detail the pre-,ana- and post-analytical flows (describe how does it happen, on what kind of samples, which machines, how many per day, how long does it take, etc.)
To identify the advantages/disadvantages of methods that will be examined.
To check the compliance with safety and hygiene measures in the laboratory (wearing gloves, aprons, etc.)
Forward, to be able to carry out a mini-audit of the field based on some points of the 15189 standard.

Learning achievement:

At the end of the course, the student will be able:

To differentiate the various fields in microbiology and their respective missions.
To understand and view the complete flow of a sample in its various fields.
To summarize the principles, advantages, disadvantages and positioning of analytical techniques used in the various fields in microbiology (microscopical, immunological, culture, molecular biology) and summarize the main steps of the development in techniques in microbiology, as well as the introduction to automation and set targets of improvements.
To explain and justify the analytical characteristics for screening, diagnostic and confirmatory tests.
To argue the importance of achieving a result rapidly.
To understand the application of ISO 15189 standard within microbiology
To contemplate an approach for the compilation and the presentation of epidemiological data and new knowledge, such as the technical ones, in a complex and evolutionary process linked with a specific infectious agent, in particular within the framework of the activities of the centers of reference and understand how to compile and present data in order to inform the population.

Kind of teaching :

Teaching is mainly based on face-to-face ex-cathedra classes during 5 hours, stimulating discussion during the lesson. The slideshows of theoretical classes are online and accessible via "my ULg."
Regarding practical exercises, students are welcome during 2 half-days in the microbiology laboratory at the CHU.
The examination will be as oral presentation in groups (3-4 students) based on practical exercises carried out in laboratory for the field chosen by students.

Learning outcomes of the learning unit

Prerequisite knowledge and skills

Planned learning activities and teaching methods

Mode of delivery (face to face, distance learning, hybrid learning)

Work placement(s)

Organisational remarks and main changes to the course

Contacts

Stéphanie Wauquier 04/323.22.90 (Stephanie.Wauquier@chuliege.be)

Association of one or more MOOCs

Items online

Clinical Microbiology - CHU Liege
Clinical Microbiology - CHU Liege

Formulaire d'audit d'un laboratoire
Formulaire d'audit d'un laboratoire

Additional biological analyses