Duration
8h Th, 20h Pr
Number of credits
| Master of Science (MSc) in Biomedical Engineering | 2 crédits | |||
| Bachelor in biomedicine | 2 crédits | |||
| Master in biology of organisms and ecology (120 ECTS) | 3 crédits |
Lecturer
Language(s) of instruction
French language
Organisation and examination
Teaching in the first semester, review in January
Schedule
Units courses prerequisite and corequisite
Prerequisite or corequisite units are presented within each program
Learning unit contents
The course is subdivided in the following way:
- cellular lines
- techniques of aseptie
- environment of the culture
- primary cultures
- techniques of observation in optical microscopy
- contaminations
- instability, variation and conservation
- measurement of viability and cytotoxicity
- three-dimensional culture
Learning outcomes of the learning unit
The philosophy of the course of culture of cells is to communicate to the students the basic techniques used for the culture of animal or human cells in vitro
The objective continued are:
- maintenance of the viable isolated cells or the functional units apart from the organism;
- application of good laboratory practices;
- handling, which constitutes the best training for the student.
This course contributes to the learning outcomes V.2 of the MSc in biomedical engineering.
Prerequisite knowledge and skills
Good knowledge in cellular and tissue biology
Planned learning activities and teaching methods
- Preparation of sterilized material and media
- Starting cells culture
- Congelation -decongelation
- Cells count
- Passaging
- Viability tests
- Observations (phase contrast microscopy)
Local: salle de TP de Microbiologie
Mode of delivery (face to face, distance learning, hybrid learning)
First semester
Course: face-to-face/podcasts
TP: in L2 Laboratory
Recommended or required readings
Notes de travaux pratiques
Dias (slideshow) on line
Ouvrage de référence: Culture of animal cells: a manual of basic technique and specialized applications. R. Ian Freshney - 7th edition - Wiley Editions (available at the library of Sciences de la Vie - CHU)
Theory: written examination in january
Work placement(s)
Organisational remarks and main changes to the course
Labcoat mandatory Laboratory book
Contacts
Dr. Erik Maquoi
Laboratoire de Biologie des Tumeurs et du Développement
Tour de Pathologie, B23 (+4), route 688
Avenue Hippocrate, 13
Sart - Tilman
B 4000 Liège
Tél: 04/366.25.31
E-mail: Erik.Maquoi@uliege.be