2017-2018 / BIOL2019-1

Theoretical chemistry and physics applied to biomoleculs structural analysis


24h Th, 24h Pr

Number of credits

 Bachelor in bioengineering4 crédits 
 Master in bioengineering : chemistry and bio-industries (120 ECTS)4 crédits 


Christian Damblon, Edwin De Pauw


Christian Damblon

Language(s) of instruction

French language

Organisation and examination

Teaching in the second semester

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

Description of the ralation between the different spectrometries.
Quantic theory and Schrödinger equation.
Ultraviolet-visible spectrometry.
Infrared and raman spectrometry.
Nuclear magnetic resonance spectrometry (1H, 13C, 2D).
Mass spectrometry.
Coupled techniques.

Learning outcomes of the learning unit

Understand theoretical basis of spectrometric methods. Apply these techniques to structural analysis of biological molécules. After completing the course the student is expected to - understand phenomenons that govern the different spectrometry : ultra-violet, visible, infrared, raman, nuclear magnetic resonance, mass spectrometry. - read and explain spectra obtained by the different techniques - apply these techniques on corresponding instruments - identify a molecule from its different spectra

Prerequisite knowledge and skills

CHIM9268-1 - General Chemistry CHIM9255-3 - Organic Chemistry CHIM9239-2 - Biological molecules chemistry CHIM9267-1 - Equilibrium Chemistry

Planned learning activities and teaching methods

Theoretical lectures.
Interpretation exercises of spectra obtained by different spectrometers. The exercises include a brief theoretical reminder with interpretation of the spectra of different chemical functions, spectrometry exercises made by students with the help of the teacher, summary exercises implementing different spectra for the same unknown molecule. Practical work for techniques of IR, MALDI-TOF, LC-MS, GC-MS and NMR. Practical work are given by sets of students working in groups. The experiments illustrate and complement the theoretical notions. Writing a report is requested at the end of the sessions. These reports will be corrected to allow the student to evaluate his work. The presence in the laboratory is mandatory. Any absence must be justified by a medical certificate in proper form. Access to the chemistry exam will not be granted to students with more than a third of unexcused absences in labs. For security reasons, access to the laboratory is authorized only for Students with a lab coat, their safety glasses and in order of registration. Glasses should be worn when handling. There is no practical work examination as such. However, questions involving laboratory situations and laboratory 'vocabulary' may appear when evaluating for exercises.

Mode of delivery (face-to-face ; distance-learning)

Lectures : 24h Practical Works : 24h (Execises 8h, practical work on devices 16h)

Recommended or required readings

The course notes include some of the literature that refers student to books that can help to better understanding of the material.  

Assessment methods and criteria

Students must bring their student Ulg card and their identity card to attend all events, under penalty of being denied access and consideration of the event. The distribution of evaluations is as follows:
- Written exam:Theory + exercices: 60% 
Practicals :40%  Attendance at practical work on devices is mandatory.

Work placement(s)

Organizational remarks


Prof. Damblon Christian                                 
Université de Liège Chimie Biologique                                     Département de Chimie                                         +32 4 3663788                               C.Damblon@ulg.ac.be
Prof. De Pauw Edwin Université de Liège Chimie Physique                                             Laboratoire de spectrométrie de masse        Département de Chimie +32 43663415 e.depauw@ulg.ac.be

Items online

Sectroscopic methods, Mass Spectrometry
Molecular structure analysis, mass spectrometry