2017-2018 / BIOL0114-3

Electronic microscopies


45h Th, 15h Pr

Number of credits

 Master in biomedical engineering (120 ECTS)5 crédits 


Philippe Compère

Language(s) of instruction

French language

Organisation and examination

Teaching in the second semester

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

The course describes the physical principles and functioning of the major instruments in modern electron microscopy. It includes the following chapters : 1) Introduction and history of electron microscopy
2) Electron-material interactions (scattering and resulting particulate and electromagnetic radiations)
3) Instrument parts (vacuum pumps, electron guns and detectors, electromagnetic lenses)
4) Instrument operation and imaging in : - scanning electron microscopy (SEM in high vacuum, low vacuum, controlled pressure conditions including "environmental" or "wet" modes - transmission electron microscopy (TEM: different image, diffraction and high resolution modes) - scanning transmission electron microscopy (STEM: principles, advantages and disadvantages)
5) Analytical electron microscopy - X-ray microanalysis (EDS) - Electron energy-loss spectroscopy(EELS) and energy filtering (EFTEM)
The course includes a practical learning on the preparation of samples for SEM/TEM as well as training sessions on the equipments of the Center of Applied Technology in Microscopy (CATµ-ULg).
This part constitutes an evaluated personnal work on a defined subjet, preferentially dedicaced to an original scientific question. 

Learning outcomes of the learning unit

Functioning principles and imaging modes in electron microscopy.
Interpretation of the images.
Influences of the imaging mode and of tunning different parameter.
Learming preparation methods for SEM/TEM in biological or material sciences accoding to the orientation of the students. 
Training as operator on SEM/TEM
Handle a short research using SEM/TEM with a good understanding of the obtained data. 

Prerequisite knowledge and skills

Bases in Physics
Master degree students in Applied Sciences and Sciences
PhD students in Applied Sciences and Sciences including the Life Sciences, Geosciences, Agronomy, Archéology,...)

Planned learning activities and teaching methods


Mode of delivery (face-to-face ; distance-learning)


Recommended or required readings

Assessment methods and criteria

Oral examination

Work placement(s)

Organizational remarks

Lectures during the 2nd period


Compère Philippe
+32 4 366 50 63