Study Programmes 2016-2017
WARNING : 2015-2016 version of the course specifications
SDOC0048-1  
Protein purification
Duration :
15h Th
Number of credits :
Doctoral training in sciences (BBMC)3
Lecturer :
Moreno Galleni, Bernard Joris, André Matagne
Coordinator :
André Matagne
Language(s) of instruction :
French language
Units courses prerequisite and corequisite :
Prerequisite or corequisite units are presented within each program
Learning unit contents :
I. Strategy (part 1); II. Theory of chromatography; III. Extraction, precipitation, concentration, dialysis ; IV. Size exclusion chromatography; V. Hydrophobic Interaction Chromatography; VI. Ion exchange; VII. Affinity chromatography; VIII. Other chromatographic methods; IX. Analytical methods for protein analysis (incl. mass spectrometry); X. Strategy (part 2); XI. Scale-up
Learning outcomes of the learning unit :
The aim of these lectures is to teach students fundamentals in protein purification methods.
Prerequisite knowledge and skills :
The language used for the lectures will be english
Planned learning activities and teaching methods :
Lectures, given with the help of powerpoint presentations, blackboard and white chalk. Furthermore, a laboratory visit will be organized to present AKTA systems.
Mode of delivery (face-to-face ; distance-learning) :
face-to-face
Recommended or required readings :
Lecture notes will be available for free (on behalf of the graduate school SFMBBM°.
Refernce book:
Scopes, R.K., Protein purification. Principles and Practice, 3rd Edition, Springer-Verlag, 1994
Assessment methods and criteria :
irrelevant
Work placement(s) :
Organizational remarks :
Students should register with Fabienne Julémont (fjulemont@ulg.ac.be) as soon as possible (program is generally available in May early).
Contacts :
André Matagne or Moreno Galleni, Centre d'Ingénierie des Protéines, Département des Sciences de la Vie, Institut de Chimie B6, Allée de la Chimie, 3, Université de Liège, B4000 Liège (Sart-Tilman)