BIOL0114-4 : Electronic microscopies

Study Programmes 2016-2017

WARNING : 2015-2016 version of the course specifications

BIOL0114-4

Electronic microscopies

Duration :

15h Th

Number of credits :

Master in physical engineering (120 ECTS)		3
Master in biology of organisms and ecology (120 ECTS)		3
Master in chemistry (120 ECTS)		2
Master in chemistry (60 ECTS)		2

Lecturer :

Philippe Compère

Language(s) of instruction :

French language

Organisation and examination :

Teaching in the second semester

Units courses prerequisite and corequisite :

Prerequisite or corequisite units are presented within each program

Learning unit contents :

The course describes the physical principles and functioning of the major instruments in modern electron microscopy. It includes the following chapters : 1) Introduction and history of electron microscopy
2) Electron-material interactions (scattering and resulting particulate and electromagnetic radiations)
3) Instrument parts (vacuum pumps, electron guns and detectors, electromagnetic lenses)
4) Instrument operation and imaging in : - scanning electron microscopy (SEM in high vacuum, low vacuum, controlled pressure conditions including "environmental" or "wet" modes - transmission electron microscopy (TEM: different image, diffraction and high resolution modes) - scanning transmission electron microscopy (STEM: principles, advantages and disadvantages)
5) Analytical electron microscopy - X-ray microanalysis (EDS) - Electron energy-loss spectroscopy(EELS) and energy filtering (EFTEM)

Learning outcomes of the learning unit :

Functioning principles and imaging modes in electron microscopy

Prerequisite knowledge and skills :

Bases in Physics
Master degree students or PhD students in sciences

Planned learning activities and teaching methods :

Lectures

Mode of delivery (face-to-face ; distance-learning) :

Face-to-face