Study Programmes 2015-2016
SDOC0048-1  
Protein purification
Duration :
15h Th
Number of credits :
Doctoral training in sciences (BBMC)3
Doctoral training in sciences (Biologie des organismes et écologie)3
Doctoral training in sciences (Chimie)3
Doctoral training in sciences (Géographie)3
Doctoral training in sciences (Géologie)3
Doctoral training in sciences (Mathématiques)3
Doctoral training in sciences (Océanographie)3
Doctoral training in sciences (Physique)3
Doctoral training in sciences (sciences et gestion de l'environnement)3
Doctoral training in sciences (sciences spatiales)3
Doctoral training in sciences (didactique des sciences)3
Lecturer :
Moreno Galleni, Bernard Joris, André Matagne
Coordinator :
André Matagne
Language(s) of instruction :
French language
Units courses prerequisite and corequisite :
Prerequisite or corequisite units are presented within each program
Course contents :
I. Strategy (part 1); II. Theory of chromatography; III. Extraction, precipitation, concentration, dialysis ; IV. Size exclusion chromatography; V. Hydrophobic Interaction Chromatography; VI. Ion exchange; VII. Affinity chromatography; VIII. Other chromatographic methods; IX. Analytical methods for protein analysis (incl. mass spectrometry); X. Strategy (part 2); XI. Scale-up
Learning outcomes of the course :
The aim of these lectures is to teach students fundamentals in protein purification methods.
Prerequisite knowledge and skills :
The language used for the lectures will be english
Planned learning activities and teaching methods :
Lectures, given with the help of powerpoint presentations, blackboard and white chalk. Furthermore, a laboratory visit will be organized to present AKTA systems.
Mode of delivery (face-to-face ; distance-learning) :
face-to-face
Recommended or required readings :
Lecture notes will be available for free (on behalf of the graduate school SFMBBM°.
Refernce book:
Scopes, R.K., Protein purification. Principles and Practice, 3rd Edition, Springer-Verlag, 1994
Assessment methods and criteria :
irrelevant
Work placement(s) :
Organizational remarks :
Students should register with Fabienne Julémont (fjulemont@ulg.ac.be) as soon as possible (program is generally available in May early).
Contacts :
André Matagne or Moreno Galleni, Centre d'Ingénierie des Protéines, Département des Sciences de la Vie, Institut de Chimie B6, Allée de la Chimie, 3, Université de Liège, B4000 Liège (Sart-Tilman)