LABO0433-2 : Modern Morphological Methods in Biomedical Research

Study Programmes 2015-2016

LABO0433-2

Modern Morphological Methods in Biomedical Research

Duration :

30h Th

Number of credits :

Master in biomedicine (120 ECTS)		3
Master in biomedicine (60 ECTS)		3

Lecturer :

Roland Greimers

Language(s) of instruction :

French language

Organisation and examination :

Teaching in the first semester, review in January

Units courses prerequisite and corequisite :

Prerequisite or corequisite units are presented within each program

Course contents :

Quantitative cytometry: individual analysis of a large number of cells by two complementary approaches including the detection of rare cells of a great biological interest (cancer cells). 1) image cytometry: presentation on all optical microscopes, confocal, phase, fluorescence, interference, electronic, and morphomathematical analysis of the images acquired by these instruments: image segmentation, binary morphometry, the watershed transformation , Fourier analysis, cell identification, counting and measurements of morphometric parameters. 2) Flow cytometry: analysis multifluorescence and high-speed sorting of thousands of cells per second. Multicolor immunofluorescence, cell cycle, calcium flux.

Learning outcomes of the course :

functionnal principles of the different apparatus and methods

Prerequisite knowledge and skills :

no prerequisite

Planned learning activities and teaching methods :

oral présentation

Mode of delivery (face-to-face ; distance-learning) :

face to face