Study Programmes 2015-2016
LABO0432-1  
Techniques for cells and tissue cultures
Duration :
5h Th, 20h Pr
Number of credits :
Master in biomedicine (120 ECTS)2
Master in biomedicine (60 ECTS)2
Lecturer :
Marie-Claire Gillet
Language(s) of instruction :
French language
Organisation and examination :
Teaching in the first semester, review in January
Units courses prerequisite and corequisite :
Prerequisite or corequisite units are presented within each program
Course contents :
The course is subdivided in the following way: - cellular lines - techniques of aseptie - environment of the culture - primary cultures - techniques of observation in optical and electronic microscopies - cloning, and selection of the cellular types - characterization - the transformed phenotype - contaminations - instability, variation and conservation - quantification and experimental projects - measurement of viability and cytotoxicity - three-dimensional culture - biocompatibility and toxicity
Learning outcomes of the course :
The philosophy of the course of culture of cells is to communicate to the students the basic techniques used for the culture of animal or human cells in vitro

The objective continued are:
- maintenance of the viable isolated cells or the functional units apart from the organism;
- application of good laboratory practices;
- handling, which constitutes the best training for the student.
Prerequisite knowledge and skills :
Good knowledge in cellular and tissue biology
Planned learning activities and teaching methods :
- Preparation of sterilized material and media - Starting cells culture - Congelation -decongelation - Cells count - Passaging - Viability tests - Observations (phase contrast and fluorescence microscopies) - Mycoplasma detection
Local: 3/62 - Institute of chemistry (B6c)
Mode of delivery (face-to-face ; distance-learning) :
First semester Course: face-to-face TP: in L2 Laboratory
Recommended or required readings :
Syllabus: Culture de cellules  - Presse universitaires de   Liège Support DVD for workshop: La culture cellulaire Notes de travaux pratiques DIAs ( showslides) on line and Presses Universitaires de  Liège
Also showslides on line
Assessment methods and criteria :
Practical :  december - Laboratory book (/20) - Exercices and questions (/20): written examination
Theory: written examination in january (/60)
Work placement(s) :
Organizational remarks :
Blouse of laboratory obligatory
Laboratory book
Contacts :
Dr M.C. De Pauw-Gillet
Institute of chemistry (B6c)
Allée de la chimie 3
Sart - Tilman
B 4000 Liège
Tél: 04/366.34.03
E-mail: Marie-Claire.DePauw@ulg.ac.be
Items online :
Basic techniques for tissue culture
Basic techniques - review
Cell Counts
Thoma  slide and Trypan Blue
Technique of cell and tissue culture
Theory
Techniques of cell and tissue culture
Slides -Course 2
Techniques of cell and tissue culture
Slides - course 3
Techniques of cell and tissue culture
Slides - course 4
Techniques of cell and tissue culture
Course 1 and 2 slides
Techniques of cell and tissue culture - practice
Dates and practice document