Study Programmes 2015-2016
| IMMU0430-1 | ||||||||
| Immunoassay | ||||||||
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Duration :
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| 5h Th, 10h Pr | ||||||||
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Number of credits :
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Lecturer :
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| Benaïssa Elmoualij | ||||||||
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Language(s) of instruction :
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| French language | ||||||||
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Organisation and examination :
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| Teaching in the first semester, review in January | ||||||||
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Units courses prerequisite and corequisite :
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| Prerequisite or corequisite units are presented within each program | ||||||||
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Course contents :
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| I-Introduction to immunological techniques
I-1. introduction I-2. Antibodies: I-3. Antigens / immunogens I-4. The antigen-antibody reaction II-Tools of mmunoassays II-1. The antibody / antigen II-2. Immunoassays: "Uses and Limitations" II-3. Hybrid systems immunoassay: aptamers, aptagènes, the affinity peptides II-4. Markers and measuring the performance of immunoassays III-New scientific and technical developments of immunoassays |
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Learning outcomes of the course :
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| This course aims to introduce the tools of immunodetection methods. The basic tools are antibodies. We will introduce the principles of immunological techniques and the choice between different strategies used to produce antibodies and other tools as an alternative will be discussed. The student will understand the current applications of immunological techniques and to propose the outline of an experimental approach to solve a problem related to the immuno-detection. | ||||||||
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Prerequisite knowledge and skills :
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| Introduction to immunology and biochemistry | ||||||||
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Planned learning activities and teaching methods :
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| The exercises are intended to illustrate the course by the simulation of immunodetections problems. Work practices illustrate some major achievements in the field chosen in different application areas: the case of aptamers. In practical work, students will learn about the influence of supports dedicated to immunodetection and perform some experiments of immunodetection through the development of ELISA and Western blot. | ||||||||
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Mode of delivery (face-to-face ; distance-learning) :
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Recommended or required readings :
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| Lectures notes
1. The immunoassay handbook. Wild, D, London : Nature Publishing Group. 2. Monoclonal antibodies : A practical approach. Oxford : Oxford University Press. 3. Immunoassays : a pratctical approach/edited by james P. Gosling. Oxford University Press. 4. Affinity and immunoaffinity purification techniques / Terry M. PHILLIPS, Benjamin F. DICKENS. Natick (Mass.) : Eaton. |
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Assessment methods and criteria :
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| Oral exam | ||||||||
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Work placement(s) :
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| Visits companies dedicated to immunodetections methods. | ||||||||
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Organizational remarks :
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Contacts :
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| Dr. Benaissa ELMOUALIJ, PhD Laboratoiry of human histology-CRPP Tour 4, 4ème étage Sart-Tilman Tél: 04/366.4328 Fax:04/3666.43.21 E-mail: b.elmoualij@ulg.ac.be | ||||||||