CHIM9245-1 : Chromatographic analysis

Study Programmes 2015-2016

CHIM9245-1

Chromatographic analysis

Duration :

12h Th, 8h Pr, 4h SEM

Number of credits :

Master in bioengineering : chemistry and bio-industries (120 ECTS)		2

Lecturer :

Stéphanie Heuskin, Georges Lognay

Coordinator :

Georges Lognay

Language(s) of instruction :

French language

Organisation and examination :

Teaching in the second semester

Units courses prerequisite and corequisite :

Prerequisite or corequisite units are presented within each program

Course contents :

- Practical aspects of chromatography (Gas liquid chromatography and high performance liquid chromatography). - Optimisation and measurement of performances, modern instrumentation and column description. Application to the chromatographic analysis of natural molecules . - Purification procedures in organic analysis including adsorption chromatography ; description of modern sampling methods for volatile products (VOCs): headspace techniques. Solid phase extraction (SPE) - Chiral chromatography.Multidimensionnal separations - Application of hyphenated techniques to the study of biomolecules. - Exercises : Case studies.
- Seminars

Learning outcomes of the course :

The applied organic chemistry course focuses on modern extraction, purification and identification methods of organic molecules from complex biological systems with special emphasize to chemical characterization, chromatographic and sampling techniques
After completing the course the student is expected to
- describe and argue about sampling and purification of the organic molecules;
- understand the different techniques (chemical analysis, derivatization, chromatography,...) for the characterization of the analytes as well as use state of the art chromatograhic methods;
- be able to discuss case studies including the theoritical aspects of the course.

Prerequisite knowledge and skills :

-- CHIM9242-4 - Fundamentals of quantitative analytical chemistry
-- CHIM9238-3 - Analytical Chemistry
-- CHIM9255-3 - Organic Chemistry
-- BIOL2017-5 - Biological Chemistry
-- BIOL2019-1 - Theoretical and physical chemistry applied to structural analysis of biomolecules

--CHIM9246-A Analytical chemistry: instrumental techniques

Planned learning activities and teaching methods :

Face-to-face
It is mandatory to attend all the seminars

Mode of delivery (face-to-face ; distance-learning) :

Lectures : 12h
Exercises: case studies 8h Seminars : 4h