CHIM9221-1 : Advanced techniques in nuclear magnetic resonance of biomolecules

Study Programmes 2015-2016

CHIM9221-1

Advanced techniques in nuclear magnetic resonance of biomolecules

Duration :

15h Th

Number of credits :

Master in chemistry (120 ECTS)		2
Master in chemistry (120 ECTS)		2
Master in chemistry (120 ECTS)		2
Master in chemistry (120 ECTS)		2

Lecturer :

Christian Damblon

Language(s) of instruction :

French language

Organisation and examination :

Teaching in the first semester, review in January

Units courses prerequisite and corequisite :

Prerequisite or corequisite units are presented within each program

Course contents :

The course is a practical application of NMR techniques studied in the course on Structural Analysis (CHIMI0650). After a lesson summarizing the theoretical principles and practicalities of different NMR experiments, students will be asked to record themselves their own experiences protein in solution interacting with ligands.
The proposed experiments are the following 1D 1H with water suppression, 2D HSQC (HN).

Learning outcomes of the course :

Objective 1: Acquiring the ability to record adequately all NMR experiments required for the analysis of biological macromolecule in water Objective 2: To understand the spectral information obtained by applying a sequence of pulses. Objective 3: to choose experiences tailored to their research.

Prerequisite knowledge and skills :

Course on structural analysis: CHIM-0213 Course on Structural biological Chemistry CHIM-0650

Planned learning activities and teaching methods :

Practical work on the NMR spectrometer. Preparation of the biological samples. Analysis of the spectra with appropriate computer programs : TOPSPIN

Mode of delivery (face-to-face ; distance-learning) :

The course schedule will be determined with the students according to the availabilities of each and the availability of the spectrometer.