Study Programmes 2015-2016
| BIOL0114-3 | ||||||||
| Electronic microscopies | ||||||||
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Duration :
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| 45h Th, 15h Pr | ||||||||
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Number of credits :
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Lecturer :
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| Philippe Compère | ||||||||
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Language(s) of instruction :
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| French language | ||||||||
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Organisation and examination :
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| Teaching in the second semester | ||||||||
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Units courses prerequisite and corequisite :
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| Prerequisite or corequisite units are presented within each program | ||||||||
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Course contents :
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| The course describes the physical principles and functioning of the major instruments in modern electron microscopy. It includes the following chapters :
1) Introduction and history of electron microscopy
2) Electron-material interactions (scattering and resulting particulate and electromagnetic radiations) 3) Instrument parts (vacuum pumps, electron guns and detectors, electromagnetic lenses) 4) Instrument operation and imaging in : - scanning electron microscopy (SEM in high vacuum, low vacuum, controlled pressure conditions including "environmental" or "wet" modes - transmission electron microscopy (TEM: different image, diffraction and high resolution modes) - scanning transmission electron microscopy (STEM: principles, advantages and disadvantages) 5) Analytical electron microscopy - X-ray microanalysis (EDS) - Electron energy-loss spectroscopy(EELS) and energy filtering (EFTEM) The course includes a practical learning on the preparation of samples for SEM/TEM as well as training sessions on the equipments of the Center of Applied Technology in Microscopy (CATµ-ULg). This part constitutes an evaluated personnal work on a defined subjet, preferentially dedicaced to an original scientific question. |
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Learning outcomes of the course :
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| Functioning principles and imaging modes in electron microscopy.
Interpretation of the images. Influences of the imaging mode and of tunning different parameter. Learming preparation methods for SEM/TEM in biological or material sciences accoding to the orientation of the students. Training as operator on SEM/TEM Handle a short research using SEM/TEM with a good understanding of the obtained data. |
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Prerequisite knowledge and skills :
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| Bases in Physics
Master degree students in Applied Sciences and Sciences PhD students in Applied Sciences and Sciences including the Life Sciences, Geosciences, Agronomy, Archéology,...) |
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Planned learning activities and teaching methods :
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| Lectures | ||||||||
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Mode of delivery (face-to-face ; distance-learning) :
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| Face-to-face | ||||||||
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Recommended or required readings :
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Assessment methods and criteria :
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| Oral examination | ||||||||
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Work placement(s) :
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Organizational remarks :
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| Lectures during the 2nd period | ||||||||
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Contacts :
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| Compère Philippe
+32 4 366 50 63 pcompere@ulg.ac.be |
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