| BIOC0210-5 | ||||||||||||||
| Functional properties of biological macromolecules | ||||||||||||||
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Duration :
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| 20h Th, 10h Mon. WS | ||||||||||||||
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Number of credits :
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Lecturer :
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| André Matagne | ||||||||||||||
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Language(s) of instruction :
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| French language | ||||||||||||||
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Organisation and examination :
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| Teaching in the first semester, review in January | ||||||||||||||
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Units courses prerequisite and corequisite :
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| Prerequisite or corequisite units are presented within each program | ||||||||||||||
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Course contents :
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| The main focus of this course is enzymology and it is organized as follows: Chapter 1: General properties of enzymes. Chapter 2: Steady-state kinetics. Chapter 3: Enzyme inhibition. Chapter 4: Effect of pH, temperature, viscosity and other experimental factors. Chapter 5: Reactions of two substrates. Chapter 6: Transient-state kinetics. Chapter 7: Cooperativity and allosteric interactions. Chapter 8: Enzyme inactivators. Chapter 9: Enzyme cofactors. Chapter 10: Mechanism of chymotrypsin. Chapter 11: Basic catalytic principles. Chapter 12: Regulation of catalytic activity. In addition some lectures might be dedicated to specific topics. This year, a lecture on the formation of disulphide bonds in proteins will be given by professor J.-F. Collet, WELBIO and de Duve Institute, U.C.L. | ||||||||||||||
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Learning outcomes of the course :
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| The aim of the course is to make students familiar with enzyme mechanisms and fundamentals of enzyme kinetics. Other topics related to the biological activity of proteins might be covered. | ||||||||||||||
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Prerequisite knowledge and skills :
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| Course on "Chemistry of biological macromolecules" (BAC3 Biology), which includes an introduction to enzyme catalysis (chapters 1-3; note that both theoretical and practical aspects are covered). | ||||||||||||||
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Planned learning activities and teaching methods :
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| Most lectures are given using the blackboard and (hopefuly) white chalk. Problems tutorials are organized in relation with some chapters. | ||||||||||||||
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Mode of delivery (face-to-face ; distance-learning) :
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| Face-to-face only | ||||||||||||||
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Recommended or required readings :
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| Lecture notes will be available following the lectures.
Reference book: A. Cornish-Bowden, Fundamentals of enzyme kinetics, fourth edition, Wiley-Blackwell, 2012. |
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Assessment methods and criteria :
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| Written exam | ||||||||||||||
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Work placement(s) :
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Organizational remarks :
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| Lectures will be organized according to the schedule known by the students. The professor will inform the students in advance of any possible modification to this | ||||||||||||||
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Contacts :
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| André Matagne, PhD, Professor, Enzymology and Protein Folding, Centre for Protein Engineering, Life Science Department, Institut de Chimie B6c (room 3/1), Allée de la Chimie, 3, University of Liège, B4000 Liège (Sart-Tilman), Tel.: +32 (0)4 3663419, Fax: +32 (0)4 3663396, Email: amatagne@ulg.ac.be | ||||||||||||||