Study Programmes 2015-2016
ANIM0008-1  
Animal cell culture
Duration :
12h Th, 8h Pr, 4h FT
Number of credits :
Master in agricultural bioengineering (120 ECTS)2
Master in agricultural bioengineering (120 ECTS)2
Master in bioengineering : chemistry and bio-industries (120 ECTS)2
Master in bioengineering : chemistry and bio-industries (120 ECTS)2
Lecturer :
Daniel Portetelle
Language(s) of instruction :
French language
Organisation and examination :
Teaching in the first semester, review in January
Units courses prerequisite and corequisite :
Prerequisite or corequisite units are presented within each program
Course contents :
Introduction: the use of animal cell culture
Biology of the cultured cells
The cell culture laboratory and its requirements
Laboratory safety and biohazards
The culture environment: substrate, gas phase, medium and temperature
Basic cell culture techniques
Quality control and biosafety of handling cells
Specialized techniques
Specific cell types and their requirements: a survey
Implications of animal cell culture in microbiology for growing of specific bacteria, virology for animal viruses, immunology and biotechnology; use in viability, cytotoxicity and modern toxicology as an alternative for animal experiment.
Learning outcomes of the course :
Overview of the basics of animal cell culture Overview of their utilization and applications in microbiology, virology, biotechnology, immunology, modern toxicology and quality control After completing the course the student is expected to - know and control in vitro culture parameters - use in vitro culture basic techniques.
Prerequisite knowledge and skills :
Concepts of cellular and molecular biology of animal cells
Planned learning activities and teaching methods :
Mode of delivery (face-to-face ; distance-learning) :
Lectures : 12h Practical and personal works : 8h Field-study trip and visits : 4h
Recommended or required readings :
Culture of animal cells (Freshney R.I.,ed.), 6°ed., 2010, Willey-Liss, New York, 576 pages Text notes and Handbook http://www.invitrogen.com/cellculturebasics.
PowerPoint available on line.
Assessment methods and criteria :
Written examination (80%) Personal report (20%)
Work placement(s) :
Organizational remarks :
Contacts :
Portetelle, Daniel (Professeur ordinaire - Chef de service) Microbiologie et Génomique 081 62 23 54
daniel.portetelle@ulg.ac.be
Items online :
Notes on line
PowerPoint on ecampus. Electronic version of articles on MyULg.