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| Version 2013-2014 |
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| VEGE0032-1 | Plant biotechnology - Molecular plant biotechnology - Exercise of plant biotechnology - Biotechnology applied to pest prevention - Biotechnology applied to phytopathology
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| Duration : | Molecular plant biotechnology : 12h Th, 6h Pr Exercise of plant biotechnology : 18h Pr Biotechnology applied to pest prevention : 9h Th, 9h Pr Biotechnology applied to phytopathology : 10h Th, 8h Pr
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| Number of credits : |
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| Lecturer : | Molecular plant biotechnology : Pierre Delaplace, Patrick du Jardin
Exercise of plant biotechnology : Pierre Delaplace
Biotechnology applied to pest prevention : Frédéric Francis
Biotechnology applied to phytopathology : Haissam Jijakli
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| Coordinator : | Patrick du Jardin |
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Language(s) of instruction :
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| French language |
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Course contents :
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 |  | Molecular plant biotechnology |

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- Genetic modification of plants : methods, techniques and applications
- New approaches for the targeted modification of genomes and their potential applications
- Genomics : methods and techniques for studying the structure and the functions of the genomes of higher plants
- Forward genetics - from phenotype to genotype - and reverse genetics - from genotype to phenotype- : advances due to molecular approaches
- The use of model plants for the understanding of the physiology of crop plants
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 |  | Exercise of plant biotechnology |

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 | Illustration of plant biotechnology use in a realistic experimental context:
- Selection of ad-hoc biotechnological tools
- Experimental set-up development
- Experimental lab work
- Data collection and analysis
- Oral presentaton of the results |
 |  | Biotechnology applied to pest prevention |

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 | Application of « omics » approches in the plant-pest relations
Biotechnological applications to control pests by using microbiological agents (entomopathogen bacteria, virus and fungi): theorical basis of action modes complemented by the production methods of these bio-insecticides
Application of transgenesis and diversity of GMO in order to control pests |
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 | Description and application of biotechnologies in the fields of biological cdontrol, diagnostics, certification and host-pathogen relations, included genetic improvement to induce resistances. |
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Learning outcomes of the course :
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 |  | Molecular plant biotechnology |

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- To understand the achievable objectives of the genetic modification technology
- To situate the GM tool in its current, developing and potential applications
- To understand the stakes and perspectives related to new tools for the targeted genetic modification of plants
- To see the point of using genomic tools to answer biological questions
- To see the point of using high throughput sequencing and plant genome annotation
- To distinguish forward and reverse genetics approaches
- To comprehend the usefulness of model organisms
- To understand the achievements and limits of studying genome expression at the transcriptomic and proteomic levels (while integrating technical and financial constraints and illustrating the use of statistical techniques suited for large datasets)
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 |  | Exercise of plant biotechnology |

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 | - To learn how to use plant biotechnology tools to address a scientific question;
- To integrate a scientific approach into an experimental set-up;
- To design a laboratory experiment and carry it out;
- Coordonated team work;
- To analyze the obtained biological dataset;
- To transmit this information to the whole student class to ensure a thorough understanding of the results. |
 |  | Biotechnology applied to pest prevention |

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 | In order to control pests and to develop alternative ways to be more sustainable, it is proposed here to describe the diversity of organisms and approaches to be potentially applied using biotechnological strategies to a diversity of targetted pests. |
 |  | Biotechnology applied to phytopathology |

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 | - To understand the bases of the exposed biotechnologies (PCR, transgenesis)
- To chose the right biotechnological tool depending on the plant poathology problem encountered (diagnostic, certification, control method, understanding of host-pathogen relations)
- To write repports in order to certify plants status |
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Prerequisites and co-requisites/ Recommended optional programme components :
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 |  | Molecular plant biotechnology |

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 | Basic courses of Genetics and Molecular Biology |
 |  | Exercise of plant biotechnology |

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 | VEGE0032-A-a |
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Planned learning activities and teaching methods :
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- Lectures ex cathedra
- Reading of scientific papers and presentation to the class
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 |  | Exercise of plant biotechnology |

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 | - Ex cathedra course : 2.5 h
- Lab work : 11.5 h
- Oral presentation (including preparation) : 4 h |
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 | Beside theorical teaching presenting the bases and action modes of the proposed biotechnological approaches, some pratical works are organised in order to apply "omics" techniques in the laboratory based on a scientific question raised on pests. |
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 | Theoretical course (6h)
Practicals in a laboratory (12h) |
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Mode of delivery (face-to-face ; distance-learning) :
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 | face-to-face |
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 | Face-to-face |
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 | Theorical teaching in auditorium
Pratical works by group in the laboratory |
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 | Face-to-face |
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Recommended or required readings :
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 |  | Molecular plant biotechnology |

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- Folder of suggested readings communicated via the online platform of the course
- Power point presentations of all lectures made available via the online platform
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 | slides, texts and papers available on line on Blackboard. |
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Assessment methods and criteria :
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- 2/3 oral examination by the board of lectures
- 1/3 : evaluation of the presentation of the articles to the class
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 |  | Exercise of plant biotechnology |

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 | - Oral presentation (...% final notation)
- Review exam in front of the teaching jury (...% final notation) |
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 | Oral examination (65%)
Report on practical works (35%) |
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Work placement(s) :
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 | All learning activities on the campus of Gembloux |
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Organizational remarks :
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 | Expected cross-links with the other courses of the module. |
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 | Organisation of this course in a collegial module - including also the evaluation |
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Contacts :
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- Patrick du Jardin (patrick.dujardin@ulg.ac.be)
- Pierre Delaplace (pierre.delaplace@ulg.ac.be)
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 | Frederic FRANCIS
Professor
Functional & evolutionary Entomology |
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 | Prof. JIJAKLI Haissam
ULg, Gembloux Agro-Bio Tech
Bât. 47, avenue Maréchal Juin
5030 Gembloux
+32 81 622431
mh.jijakli@ulg.ac.be |
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| Items online : |
Molecular plant biotechnology
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| Course contents |
| All the pedagogical resources used in this course are downloadable from the corresponding folder on the online teaching platform eCampus. |
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Exercise of plant biotechnology
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| Course contents |
| All the pedagogical ressources used in this course are downloadable from the corresponding folder on the online teaching platform eCampus. |
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