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| LABO0432-2 | Techniques for cells and tissue cultures
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| Duration : | 30h Th |
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| Number of credits : |
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| Lecturer : | Marie-Claire Gillet |
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Language(s) of instruction :
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| French language |
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Organisation and examination :
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| Teaching in the first semester, review in January |
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Course contents :
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| The course is subdivided in the following way:
- cellular lines
- techniques of aseptie
- environment of the culture
- primary cultures
- techniques of observation in optical and electronic microscopies
- cloning, and selection of the cellular types
- characterization
- the transformed phenotype
- contaminations
- instability, variation and conservation
- quantification and experimental projects
- measurement of viability and cytotoxicity - three-dimensional culture
- biocompatibility and toxicity |
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Learning outcomes of the course :
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| The philosophy of the course of culture of cells is to communicate to the students the basic techniques used for the culture of animal or human cells in vitro
The objective continued are:
- maintenance of the viable isolated cells or the functional units apart from the organism;
- application of good laboratory practices;
- handling, which constitutes the best training for the student. |
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Prerequisites and co-requisites/ Recommended optional programme components :
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| Good knowledge in cellular and tissue biology |
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Planned learning activities and teaching methods :
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| - Preparation of sterilized material and media
- Starting cells culture
- Congelation -decongelation
- Cells count
- Passaging
- Viability tests
- Observations (phase contrast and fluorescence microscopies)
- Mycoplasma detection
Local: 3/62 - Institute of chemistry (B6c) |
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Mode of delivery (face-to-face ; distance-learning) :
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| First semester
Course: friday
19 september, 08h30-10h30 (Sternon)
26 september, 13h30-15h30 (Rév. Micro.)
3 october, 13h30-15h30 (D50)
10 october, 8h30-10h30 (F50)
TP: thursday - friday
from the week 8
6 november until until 12 december
NB: to be modified in function of the number of students and the teaching duties
Autonomy- examination: december |
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Recommended or required readings :
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| Syllabus: Culture de cellules - Presses universitaires de Liège
Support DVD for workshop: La culture cellulaire
Notes de travaux pratiques
DIAs ( power point presentation) Presses universitaires de Liège
Cours and notes also on line |
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Assessment methods and criteria :
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| Practical :
- Laboratory book (/20) - 15 december
- Workshop (/20) - december
Theory: written examination in january (/60)
To be modified in function of the teaching duties |
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Work placement(s) :
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Organizational remarks :
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| Blouse of laboratory obligatory
Laboratory book |
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Contacts :
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| Dr M.C. De Pauw-Gillet
Institute of chemistry (B6c)
Allée de la chimie 3
Sart - Tilman
B 4000 Liège
Tél: 04/366.34.03
E-mail: Marie-Claire.DePauw@ulg.ac.be |
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