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| Version 2013-2014 |
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| IMMU0430-1 | Immunoassay
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| Duration : | 5h Th, 10h Pr |
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| Number of credits : |
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| Lecturer : | Benaïssa Elmoualij |
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Language(s) of instruction :
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| French language |
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Course contents :
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| I-Introduction to immunological techniques
I-1. introduction
I-2. Antibodies:
I-3. Antigens / immunogens
I-4. The antigen-antibody reaction
II-Tools of mmunoassays
II-1. The antibody / antigen
II-2. Immunoassays: "Uses and Limitations"
II-3. Hybrid systems immunoassay: aptamers, aptagènes, the affinity peptides
II-4. Markers and measuring the performance of immunoassays
III-New scientific and technical developments of immunoassays |
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Learning outcomes of the course :
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| This course aims to introduce the tools of immunodetection methods. The basic tools are antibodies. We will introduce the principles of immunological techniques and the choice between different strategies used to produce antibodies and other tools as an alternative will be discussed. The student will understand the current applications of immunological techniques and to propose the outline of an experimental approach to solve a problem related to the immuno-detection. |
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Prerequisites and co-requisites/ Recommended optional programme components :
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| Introduction to immunology and biochemistry |
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Planned learning activities and teaching methods :
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| The exercises are intended to illustrate the course by the simulation of immunodetections problems. Work practices illustrate some major achievements in the field chosen in different application areas: the case of aptamers.
In practical work, students will learn about the influence of supports dedicated to immunodetection and perform some experiments of immunodetection through the development of ELISA and Western blot. |
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Mode of delivery (face-to-face ; distance-learning) :
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Recommended or required readings :
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| Lectures notes
1. The immunoassay handbook. Wild, D, London : Nature Publishing Group.
2. Monoclonal antibodies : A practical approach. Oxford : Oxford University Press.
3. Immunoassays : a pratctical approach/edited by james P. Gosling. Oxford University Press.
4. Affinity and immunoaffinity purification techniques / Terry M. PHILLIPS, Benjamin F. DICKENS. Natick (Mass.) : Eaton. |
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Assessment methods and criteria :
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| Oral exam |
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Work placement(s) :
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| Visits companies dedicated to immunodetections methods. |
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Organizational remarks :
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Contacts :
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| Dr. Benaissa ELMOUALIJ, PhD
Laboratoiry of human histology-CRPP
Tour 4, 4ème étage
Sart-Tilman
Tél: 04/366.4328
Fax:04/3666.43.21
E-mail: b.elmoualij@ulg.ac.be |
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