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| GENE0001-4 | Genetic engineering
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| Duration : | 20h Th |
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| Number of credits : |
| Master in Biochemistry and Molecular and Cell Biology, Research Focus, 1st year | | 2 |
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| Master in Biochemistry and Molecular and Cell Biology, Teaching Focus, 1st year | | 2 |
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| Master en biochimie et biologie moléculaire et cellulaire, à finalité spécialisée en bio-industrie, 1st year | | 2 |
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| Master in Biochemistry and Molecular and Cell Biology, Professional Focus in
Industrial Biochemistry, 1st year | | 2 |
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| Master en biochimie et biologie moléculaire et cellulaire, à finalité spécialisée en bioinformatique et modélisation, 1st year | | 2 |
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| Master in Biological Sciences | | 2 |
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| Lecturer : | Jacques Dommes |
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Language(s) of instruction :
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| French language |
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Organisation and examination :
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| Teaching in the first semester, review in January |
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Course contents :
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| 1. Introduction
- Definitions
- Basics of molecular biology
- Genome sizes
2. Manipulation of DNA fragments
- Restriction enzymes
- Enzymatic synthesis of DNA
- Chemical systhesis of DNA
- Ligation of DNA fragments
- DNA labeling
- Separation of DNA fragments
3. Genetic engineering of Prokaryotes
- Plasmid vectors
- Lambda phage vectors
- Cosmids
- Vectors for in vitro transcription
- Expression vectors for the production of heterologous proteins in E. coli
- Expression of recombinant proteins in other bacteria
4. Construction and screening of DNA libraries
- Number of clones in a DNA library
- Construction of a DNA library
- Screening of a DNA library by hybridisation
- Screening of a DNA library constructed in an expression vector
5. Site-directed mutagenesis
- Random mutagenesis of a gene
- Mutagenesis by DNA fragment exchange
- Primer elongation
- Mutagenesis by PCR
6. Specific application of PCR
- Contaminations in PCR
- PCR-ELISA
- Quantitative PCR and RT-PCR
- In situ PCR
- Cloning PCR products
- Asymetric PCR
- Nested PCR
7. Genetic engineering of yeasts and filamentous fungi
- Introduction
- Transformation methods in yeasts
- Vectors for Saccharomyces cerevisiae
- Promoters for S. cerevisiae
- Secretion of the recombinant protein
- Other yeasts and fungi
- The two-hybrid system |
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Learning outcomes of the course :
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| · To get acquainted with the main methods used in genetic engineering
· To get informed of the potential of genetic engineering in the study of living organisms, as well as in the improvement of species for human use and for the production of recombinant proteins. |
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Prerequisites and co-requisites/ Recommended optional programme components :
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| Molecular biology and genetics (courses of the bachelier in Biology). |
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Planned learning activities and teaching methods :
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Mode of delivery (face-to-face ; distance-learning) :
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| Lectures during first period. |
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Recommended or required readings :
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| Powerpoint presentations and lecture texts available through the eCampus system.
The following book may be interesting but not necessary :
Principles of Gene Manipulation and Genomics, S.B. Primrose and R.M. Twyman, 7th edition, Blackwell, ISBN13 : 978-1-4051-3544-3 |
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Assessment methods and criteria :
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| Oral exam (first exam exam session, as well as second one). |
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Work placement(s) :
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Organizational remarks :
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Contacts :
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| - Prof. J. Dommes (j.dommes@ulg.ac.be) |
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| Items online : |
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| Course materials |
| available on eCampus |
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